Moss, C. Wayne (North Carolina State University, Raleigh), and M. L. Speck. Release of biologically active peptides from Escherichia coli at subzero temperatures. J. Bacteriol. 91:1105-1111. 1966.-Freezing and storage of Escherichia coli at -20 C in phosphate buffer resulted in loss of cell viability and a pronounced leakage of cellular material which had maximal absorption at 260 mmu. Greater ...

Accurate, precise and reproducible isocratic RP-HPLC method was developed and subsequent validated for the analysis of Torasemide (I), Irbesartan (II) and Olmesartan medoxomil (III) at ambient temperature, using Atlantis 4.6 mm x 250 mm RP-C18 Column, with a flow rate of 1.5 ml.min, and UV. detector at 288 nm and 260 nm for (I) and (II and III), respectively. By adopting the mentioned chromatog...

2Department of Physiology, School of Pharmaceutical SciencesUniversiti Sains Malaysia, 11800 Minden, Penang, Malaysia. A b s t r a c t The purpose of this research was to study of physicochemical properties of the extracts and to develop and validate HPLC method in order to study their solubility in organic and aqueous vehicles and partition coefficients. The physicochemical characteristics suc...

A rapid and simple liquid chromatographic method was developed for the quantitative determination of dexrabeprazole sodium in tablet dosage form. The reverse-phase analysis carried out by using a C-18 column. acetonitrile-phosphate buffer mixture used as mobile phase. obtained retention time 4.33∓0.02 min. 50:50 (v:v) acetonitrile:phosphate buffer(pH 7) 1.0 mL/min. flow rate. Quantification ana...

The phosphate transport protein was purified from rat liver mitochondria by extraction in an 8% (v/v) Triton X-100 buffer followed by adsorption chromatography on hydroxyapatite and Celite. SDS/polyacrylamide-gel electrophoresis (10%, w/v) demonstrated that the purified polypeptide was apparently homogeneous when stained with Coomassie Blue and had a subunit Mr of 34000. However, lectin overlay...

In traditional cell-free protein synthesis reactions, the energy source (typically phosphoenolpyruvate (PEP) or creatine phosphate) is the most expensive substrate. However, for most biotechnology applications glucose is the preferred commercial substrate. Previous attempts to use glucose in cell-free protein synthesis reactions have been unsuccessful. We have now developed a cell-free protein ...

A constitutive alkaline phosphatase was purified to apparent homogeneity as determined by polyacrylamide gel electrophoresis from mycelia of the wild strain 74A of the mold Neurospora crassa, after growth on acetate and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 h at 30 degrees C. The molecular mass was 58 kDa and 56 kDa as determined by exclusion chromatography an...

Recently, dynamic approach has been applied to determine the steady state concentrations of multiple ionic species present in complex buffers at equilibrium. Here, we have used the dynamic approach to explicitly model the pH profiles of biologically relevant phosphate buffer and universal buffer (a mixture of three tri-protic acids such as citric acid, boric acid and phosphoric acid). The resul...

Titanium dioxide (TiO2) or titania shows great promise in detoxification and drug delivery. To reach its full potential, it is important to interface TiO2 with biomolecules to harness their molecular recognition function. To this end, DNA attachment is an important topic. Previous work has mainly focused on long double-stranded DNA or single nucleotides. For biosensor development and targeted d...

The effect of HbA and HbF on both the aggregation and deaggregation of deoxy HbS and deoxy HbCHarlem in concentrated phosphate buffers was studied by a turbidimetric method. Although pure deoxy HbA is fully soluble in 2.4 M potassium phosphate buffer, the same concentration of deoxy HbAS was insoluble in the same buffer. Since there was little dissolved hemoglobin in the solute of a mixture of ...