Immobilized metal ion affinity chromatography principles were applied for selective immobilization of recombinant polyhistidine tag fused phenylalanine ammonia-lyase from parsley (PcPAL) on porous polymeric support with aminoalkyl moieties modified an EDTA dianhydride (EDTADa)-derived chelator and charged transition ions. Out the five investigated ions - Fe3+, Co2+, Ni2+, Cu2+, Zn2+ best biocat...

Compounds with planar triple ring systems such as acridine orange, 9-amino acridine, 9-amino-1,2,3, 4-tetrahydroacridine (tacrine), 6,9-diamino-2-ethoxyacridine lactate monohydrate (DE-acridine), 6-chloro-9-(3'-diethylamino-2'-hydroxypropylamino)-2-methoxyacridine 2 HCI (CDM-acridine), quinacrine, 6-chloro-9(4'diethylamino1'-methylbutylamino) .2-methoxy. 1, 10-diazaanthracene (CDM 1, 10-diazaan...

Banana blossoms are rich in fiber and nutrients a popular plant-based, vegan alternative to fish. However undesirable browning, usually visible at the peduncle cut-end, negatively impacts consumer acceptability of banana blossoms. The aim this work was develop safe alternatives prevent browning First, activities primary enzymes associated with tissue i.e., polyphenol oxidase (PPO), peroxidase (...

Abstract Nitric oxide (NO) is a highly reactive gaseous small molecule that regulates plant growth. Sodium nitroprusside (SNP) was used as an NO donor to study the effects of treatment on browning and antioxidant activity button mushrooms ( Agaricus bisporus ). The findings revealed exogenous alleviated browning, decreased hardness, increased weight loss stored at 4°C for 15 days, reduced accum...

An efficient enzymatic process was developed to produce optically pure D-phenylalanine through asymmetric resolution of the racemic DL-phenylalanine using immobilized phenylalanine ammonia-lyase (RgPAL) from Rhodotorula glutinis JN-1. RgPAL was immobilized on a modified mesoporous silica support (MCM-41-NH-GA). The resulting MCM-41-NH-GA-RgPAL showed high activity and stability. The resolution ...

The enzyme histidine ammonia-lyase (histidase) is required for growth of Streptomyces griseus on L-histidine as the sole source of nitrogen. Histidase was induced by the inclusion of histidine in the medium, regardless of the presence of other carbon and nitrogen sources. Histidase activity was increased by a shift of culture incubation temperature from 30 to 37 degrees C. Conversely, upon indu...

aril browning threatens production, consumption, and exports of pomegranates, because affected fruit cannot be externally distinguished from healthy fruit. this study compared the mineral, biochemical composition, and related enzyme activities in affected brown arils with healthy ones in ‘malase saveh’ pomegranates. the results indicated that concentrations of cu in the aril and k, mg, and mn i...

In the present study, the effect of exogenous silver nitrate (Ag+) and copper sulfate (Cu2+) applications on production of artemisinin and phenolic compounds as well as oxidative stress in the shoot cultures of Artemisia annua was investigated. A significant decrease in the shoot biomass and the total chlorophyll was observed in the shoots exposed to increased Ag concentration. Additionally, a ...

salicylic acid (sa) and methyl jasmonate (meja) as phytohormone regulate synthesis of secondary metabolites in a wide range of plant species thereby accumulation of phenylalanine ammonia lyase enzyme (pal) and accelerate of oxygen free radicals, protein kinas and no production. the present research designed to evaluate effect of sa and meja elicitation on accumulation of phenolic compounds, fla...