Poly-beta-hydroxybutyrate (PHB) can be formed in large amounts in Cupriavidus necator and is important for the industrial production of biodegradable plastics. In this investigation, laser tweezers Raman spectroscopy (LTRS) was used to characterize dynamic changes in PHB content-as well as in the contents of other common biomolecule-in C. necator during batch growth at both the population and s...

Background & objective: Globally, breast cancer is the most common malignancy among females. Prohibition (PHB)-I, a homologous protein, was initially introduced as a suppressor gene for amplification process. Further, the protein has a key role in the cell cycle and is capable of inhibiting DNA transcription in many cell types. Therefore, its possible role in different...

We assessed the effects of different arcA mutations on poly(3-hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis...

A statistically based Plackett-Burman screening design identified milk whey and corn steep liquor concentrations as well as ionic strength (based on phosphate buffer concentration) as the three main independent components of the culture medium that significantly (p < 0.05) influenced biomass and poly(3-hydroxybutyrate) (PHB) production in recombinant cells of Escherichia coli. This strain carri...

Molecular methods have been applied to analyze the expression of the Alcaligenes eutrophus poly(3-hydroxybutyrate) (PHB) synthase gene (phbC). The translational initiation codon was identified by analysis of the amino acid sequence of a PHB synthase-beta-galactosidase fusion protein. This protein was purified to almost gel electrophoretic homogeneity by chromatography on DEAE-Sephacel and on am...

Poly-f-hydroxybutyric acid (PHB) has been demonstrated in a wide range of bacteria, including both chemolithotrophic and photolithotrophic organisms. In the chemolithotrophic group, Schlegel, Gottschalk, and von Bartha (8) reported the formation and utilization of PHB in Hydrogenomonas, Lundgren et al. (4) extracted PHB from Ferrobacillusferrooxidans, and Tobback and Laudelout (10) chemically i...

Poly(3-hydroxybutyrate) (PHB) has been proposed to be a potential candidate used as biomaterial. However, its poor processability, high brittleness and rigidity have limited applicability. Transesterification reactions with poly(e-caprolactone) (PCL), one of the most promising biomedical materials, emerge an attractive alternative improve mechanical properties. In this work, poly(3-hydroxybutyr...

Introduction: Gated SPECT myocardial perfusion scanning has new capabilities in addition to its main applications such as left ventricular dyssynchrony using phase analysis. Phase analysis has been investigated through various software including Emory Cardiac Toolbox (ECTb) and Quantitative Gated SPECT (QGS). The aim of this study is to evaluate the effect of reconstruction par...

BACKGROUND Poly(3-hydroxybutyrate) (PHB), have been considered to be good candidates for completely biodegradable polymers due to their similar mechanical properties to petroleum-derived polymers and complete biodegradability. Escherichia coli has been used to simulate the distribution of metabolic fluxes in recombinant E. coli producing poly(3-hydroxybutyrate) (PHB). Genome-scale metabolic net...

A quantitatively repeatable protocol was developed for poly(3-hydroxybutyrate) (PHB) production by Escherichia coli XL1-Blue (pSYL107). Two constant-glucose fed-batch fermentations of duration 25 h were carried out in a 5-L bioreactor, with the measured oxygen volumetric mass-transfer coefficient (k(L)a) held constant at 1.1 min(-1). All major consumption and production rates were quantified. T...