Exploiting the innate hydrolytic enzyme/s potential of the genus Bacillus for costeffective production of PHAs, using various agro-industrial wastes will contribute to cost reduction and elimination of pre-treatment protocols. PHA producing Bacillus spp. from different rhizospheric soils capable of producing a variety of hydrolytic enzymes were isolated. Two promising isolates: Ti1 and Ti3, fro...

Previous research has demonstrated that post-anoxic denitrification and biological nutrient removal could be achieved in the oxic/anoxic/extended-idle wastewater treatment regime. This study further investigated the effect of different carbon sources on post-anoxic denitrification and biological nutrient removal. Acetate, propionate (volatile fatty acids (VFAs)), glucose (carbohydrate), methano...

In this study, it was possible to evaluate the production of polyhydroxyalkanoates (PHAs) from microalgae Chlorella vulgaris and Tetradesmus obliquus, using 1% 0.25% (v/v) agro-indusytial Corn Steep Liquor residue, a product corn processing. The biomass subjected mechanical extraction 4% sodium hypochlorite + chloroform, extract further characterized Fourier Transform Infrared Spectroscopy (FTI...

Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant m...

We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800-1800 cm-1) in...

Dissection of the genetic pathways and mechanisms by which anther development occurs in grasses is crucial for both a basic understanding of plant development and for examining traits of agronomic importance such as male sterility. In rice, MULTIPLE SPOROCYTES1 (MSP1), a leucine-rich-repeat receptor kinase, plays an important role in anther development by limiting the number of sporocytes. OsTD...