We characterized two bacteriophages, ΦFG02 and ΦCO01, against clinical isolates of Acinetobacter baumannii established that the bacterial capsule is receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible biosynthesis, resulting loss disruption phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam an...

In this paper, we present a provably secure redactable signature scheme allowing to independently redact structure and content. We identify the problems when structure is not separated from content, resulting in an attack on the scheme proposed at VLDB ’08 by Kundu and Bertino. The attack allows for changing the semantic meaning of a given tree. We introduce a rigid security model, including co...

Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an...

Three individual peptide sequences, EVSHPKVG, WVTTSNQW, and SGGSNRSP, which have potentials to bind to Newcastle disease virus (NDV), were identified by the biopanning method using phage display technology. The binding specificities of these peptides presented on phages were confirmed by ELISA competition assay using chicken anti-NDV antiserum. The synthetic peptides designed based on these res...

33 stools from 518 patients suspected of having cholera were examined. from 174 of these patients vibrio ei tor was isolated. po of these strains belonged to phage type iv, 53 to phage type v and one strain was untypable. it is suggested that these strains originated from two different sources.

bacteriophage is a kind of virus that infect bacteria, and is distinct from the animal and plant viruses that could have either lytic or lysogenic cycle. lytic phages known as candidates for phage therapy, since they rapidly replicate into their host and lyse them. theoretically, phages have more than a few benefits over routine antibiotics. they are extremely specific and for their targeted ho...

Abstract Plasmids and temperate phages are key contributors to bacterial evolution. They usually regarded as very distinct. However, some elements, termed phage–plasmids, known be both plasmids phages, e.g. P1, N15 or SSU5. The number, distribution, relatedness characteristics of these phage–plasmids poorly known. Here, we screened for elements among ca. 2500 12000 identified 780 across diverse...

The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin Acceptor Peptide tag (BAP; also known as AviTag) is effected on a specific lysine either by co-expressing the E. coli ...

The deeply intertwined evolutionary history between bacteriophages and bacteria has endowed phages with highly specific mechanisms to hijack bacterial cell metabolism for their propagation. Here, we present a comprehensive, phage-driven strategy reveal novel antibacterial targets by the exploitation of phage-bacteria interactions. This will enable design small molecules, which mimic inhibitory ...

BACKGROUND Mycobacterium avium subspecies paratuberculosis (MAP) is a versatile pathogen with a broad host range. Its association with type-1 diabetes mellitus (T1DM) has been recently proposed. Rapid identification of infectious agents such as MAP in diabetic patients at the level of clinics might be helpful in deciphering the role of chronic bacterial infection in the development of autoimmun...