A protein fraction which inhibits growth of weanling rats was obtained from Phaseolus vulgaris seeds of the Sanilac variety by extraction with sodium chloride solution and removal of extraneous materials by precipitation at pH 4.0 and absorption on I : I bentonite-Celite. The protein was precipitated by 0.75 saturation with ammonium sulfate. The growth inhibiting protein fraction contained at l...

background and objectives : streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of streptococcus equisimilis strain h46a. the objective of the present study was the production of streptokinase from strain h46a and purification by chemical reduction method. materials and methods: the rate of...

Vanadium-modified (SiO2/MgO/MgCl2)•TiClx Ziegler-Natta catalysts were prepared through co-impregnation of water-soluble magnesium and vanadium salts under different pH values. Several key factors such as pH value of co-impregnation solution during catalyst preparation, catalyst performances including catalytic activity, ethylene/1-hexene copolymerization and hydrogen response were investigated....

The nature of the encephalopathic agent present in monkey bone marrow has been investigated. It is readily soluble at a pH of 7.4, as well as at the low pH of 2.0 and 3.0. The point of minimal solubility is pH 4.2. Heat inactivation is produced by a temperature of 75 degrees C., acting for 15 minutes. The agent is digested by pepsin. It exhibits a slight degree of sedimentation with the air-dri...

The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquidliquid extraction of proteins. We investigated the solubilization of cytochrorne-c and 0-chymotrypsin into the NaDEHP reverse micellar phase by varying the pH and NaCl concentration in the aqueous phase. At neutral pH and relatively low ionic strength, the proteins are extracted into th...

In this study, in-house extracted kidney bean protein concentrate (KPC) was treated with pH-shift (pH-S), combined ultrasound (pH-SU) or heat (pH-SH), and the structural, functional, interfacial properties of modified KPC were comprehensively evaluated. Upon treatments, increase surface hydrophobicity (H0) observed, intensity in order pH-SH > pH-SU pH-S control. The intrinsic tryptophan fluores...

Reversible voltammograms and a voltammetry half-wave potential versus solution pH diagram are described for a protein tyrosine radical. This work required a de novo designed tyrosine-radical protein displaying a unique combination of structural and electrochemical properties. The α(3)Y protein is structurally stable across a broad pH range. The redox-active tyrosine Y32 resides in a desolvated ...