Ultraviolet B (UVB) irradiation generates reactive oxygen species (ROS), which can damage exposed skin cells. Mitochondria and NADPH oxidase are the two principal producers of ROS in UVB-irradiated keratinocytes. Peroxiredoxin V (PrxV) is a mitochondrial cytosolic cysteine-dependent peroxidase enzyme that robustly removes H2O2. We investigated PrxV’s role protecting epidermal keratinocytes agai...

Production of reactive oxygen species (ROS) and protein state membrane-cytoskeleton complex (MCC) in the erythrocytes, subjected to freeze-thawing with polyethylene glycol (PEG) subsequently transferred into physiological conditions vitro after cryoprotective agent removal were examined. Results SDS-PAGE β-mercaptoethanol showed a decrease spectrin content MCC profile cryopreserved erythrocytes...

Mitotane, 1,1-dichloro-2-(o-chlorophenyl)-2-(p-chloro-phenyl) ethane (o,p'-DDD), is a compound that represents the effective agent in the treatment of the adrenocortical carcinoma (ACC), able to block cortisol synthesis. In this type of cancer, the biological mechanism induced by this treatment remains still unknown. In this study, we have already shown a greater impairment in the first steps o...