Background Vero cells are adherent cell lines commonly used for the production of viral vaccines. We had developed an animal component free medium that allows an optimal growth of this cell line in stirred bioreactor [1,2]. We had also showed that Vero cells grown in this medium (called IPT-AFM) sustained rabies virus replication, and resulted in an overall yield comparable to the level obtaine...

The statements of Castellani and Chalmers (1919) and Bergey (1934) that the Bacteroides show good growth on ordinary laboratory media have been quite misleading, for difficulty of cultivation has been among the most trying problems encountered in working with the non-sporulating anaerobic bacteria. Harris (1901-1905) and Beaver, Henthorne and Macy (1934) have especially emphasized this point, a...

Until the present time it has been proven impossible to isolate as pure chemical individuals any of the reactants concerned in the production of light by organisms. The work of Dubois 1 and Harvey has, however, indicated the general nature of such reactions as consisting in the oxidation of a substrate, luciferin, catalyzed by an enzyme, luciferase. Harvey~ believes that luciferin should be pla...

A new gram-negative, non-sporulating, mesophilic, amino acid fermenting bacterium, designated strain ALA-1(T) (T = type strain), was isolated from an anaerobic lagoon of a dairy wastewater treatment plant. The strain is curved (3-4 microm x 0.2-0.3 microm) and occurs singly or in pairs. Optimum growth occurs at 37 degrees C and pH 7.3. The G+C content of the DNA is 46 mol %. The strain requires...

The constituents of an enzymatic hydrolysate of protein can be divided according to their complexit,y into six fractions; protein, metaprotein, proteose, peptone, subpeptones, and amino acids. A method for the quantitative estimation of these fractions was devised in order to secure more definite information regarding the changes occurring during hydrolysis than is obtained by the usual free am...

IT has been suggested that the refinement of staple carbohydrate foods may be a major aetiological factor in accounting for the appearance and the rising incidence of duodenal ulcer, both in Western countries and in developing countries, during the present century (Cleave, 1962, 1974). Cleave postulated that this may be due to loss of buffer in the staple carbohydrates as a result of the remova...