1. An in vitro method which used enzymic digestion of the food matrix to release biologically available vitamin B6 is described. 2. Vitamin B6-fortified liquid model foods were thermally processed. After these foods had been freeze-dried, one part was subjected to enzymic hydrolysis at pH 2.0 with pepsin (EC 3.4.23.1) followed by a hydrolysis at pH 8.0 with pancreatin. The vitamins that were fo...

the aim of this study was to evaluate the sensitivity of the population of fez and casablanca in morocco to dry white beans (phaseolus vulgaris) and to investigate the effect of food processing (heat and/or enzymatic hydrolysis by pepsin) on this sensitivity. work was based on a bank consisting of 146 sera from patients with atopic hypersensitivity in order to evaluate specific immunoglobulin e...

The specificity of pepsin, the major protease gastric digestion, has been previously investigated, but only regarding primary sequence protein substrates. present study aimed to consider in addition physicochemical and structural characteristics, at molecular sub-molecular scales. For six different proteins submitted vitro peptide bonds cleaved were determined from peptides released identified ...

Hempseed meal after protein isolation (HM-PI) is a co-product obtained from hempseed. The objectives were to characterize and determine the effect of drying on HM-PI. HM-PI was produced using three methods: freeze (FD), vacuum oven (VOD), (OD). contained over 70% had similar or higher level essential amino acids than recommended values for human adults. Osborne fractionation indicated that glut...

Sera from two blood donors, one of whom was implicated in a case of post-transfusion hepatitis B, were found to be positive for anti-HBc and negative for HBsAg by conventional radioimmunoassay and were retested for HBsAg after concentration (pepsin digestion and polyethylene glycol precipitation). The presence of occult HBsAg was confirmed in both. These observations have implications for blood...

Sera from two blood donors, one of whom was implicated in a case of post-transfusion hepatitis B, were found to be positive for anti-HBc and negative for HBsAg by conventional radioimmunoassay and were retested for HBsAg after concentration (pepsin digestion and polyethylene glycol precipitation). The presence of occult HBsAg was confirmed in both. These observations have implications for blood...

A quantitative method is described which permits a determination of the relative amount of trypsin or pepsin present in a gelatin-enzyme digestion mixture, provided the gelatin and trypsin solutions are purified. This method is dependent upon the change in viscosity of such solutions. It is found that the time required to cause a given percentage change in the viscosity is nearly inversely prop...

Electron microscope examination of the adrenal cortex from three male human subjects revealed a special type of cell occurring in periendothelial spaces, in all adrenal cortex zones. It is a clear, spindle-shaped cell the principal cytoplasmic features of which are crystalline inclusions with a structure similar to that of the Reinke crystals of human testicular interstitial cells and an abunda...

The complete amine acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleayed with cyanogen bromide and the resulting three fragments, CBI, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CBI were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin ef reduced SLcarboxymethylated ...