background pcr is a molecular technique for herpes simplex virus (hsv) detection that can cause life-threatening infections such as encephalitis and keratitis. however, the main issues, false-negative results causing by pcr inhibitors, of this technique that reduce pcr efficiency. to overcome this problem, a competitive internal amplification control (iac) was constructed for conventional pcr u...

Aim: To compare the results of DNA amplification by the polymerase chain reaction (PCR) with immunofluorescence staining for detecting Pneumocystis carin'i in bronchoalveolar lavage specimens taken from symptomatic HIV seropositive patients with suspected P carinii pneumonia (PCP). Methods: Bronchoalveolar lavage specimens were obtained from 28 symptomatic HIV seropositive patients. Specimens w...

AIM To compare the results of DNA amplification by the polymerase chain reaction (PCR) with immunofluorescence staining for detecting Pneumocystis carinii in bronchoalveolar lavage specimens taken from symptomatic HIV seropositive patients with suspected P carinii pneumonia (PCP). METHODS Bronchoalveolar lavage specimens were obtained from 28 symptomatic HIV seropositive patients. Specimens w...