نتایج جستجو برای: pcr reflp

تعداد نتایج: 175063  

Journal: :Current issues in molecular biology 2013
David Rodriguez-Lazaro Nigel Cook Marta Hernandez

A principal consumer demand is a guarantee of the safety and quality of food. The presence of foodborne pathogens and their potential hazard, the use of genetically modified organisms (GMOs) in food production, and the correct labelling in foods suitable for vegetarians are among the subjects where society demands total transparency. The application of controls within the quality assessment pro...

Journal: :BioTechniques 1997
M Fille J D Shanley J Aslanzadeh

One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...

Journal: :Angewandte Chemie 2005
Haikuo Li Jiehuan Huang Junhong Lv Hongjie An Xiaodong Zhang Zhizhou Zhang Chunhai Fan Jun Hu

The polymerase chain reaction (PCR) revolutionized molecular genetics and has become one of the most popular techniques in modern biological and medical sciences. Owing to the exponential amplification ability of PCR, one could start from even a single copy of target DNA to produce large amounts of DNA copies for sequencing, molecular diagnosis, or genetic analysis. This remarkable amplificatio...

Journal: :Epigenetics 2009
Tomasz K Wojdacz Tanni Borgbo Lise Lotte Hansen

Many protocols in methylation studies utilize one primer set to generate a PCR product from bisulfite modified template regardless of its methylation status (methylation independent amplification MIP). However, proportional amplification of methylated and unmethylated alleles is hard to achieve due to PCR bias favoring amplification of unmethylated relatively GC poor sequence. Two primer design...

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