نتایج جستجو برای: pcr multiplex

تعداد نتایج: 186945  

2010
Nguyen Thi Thanh Thao Nguyen Thi Kim Ngoc Phan Văn Tú Trần Thi Thúy Mary Jane Cardosa Peter Charles McMinn Patchara Phuektes

Human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) are two major aetiological agents of hand, foot and mouth disease (HFMD) in children. Recently there have been several large outbreaks of HFMD in Vietnam and the Asia-Pacific region. In this study, a multiplex RT-PCR assay was developed in order to detect simultaneously HEV71, CVA16 and other human enteroviruses. Enterovirus detection ...

Journal: :BioTechniques 1997
J G Chang H J Liu J M Huang T Y Yang C P Chang

A rapid and simple method, termed multiplex mutagenically separated PCR (MS-PCR), was developed to detect several molecular defects in the hemoglobin gene in one PCR. This technique, in which different-size allele-specific primers were used, specifically amplified both normal and mutant alleles of the globin gene in the same reaction. Subsequent gel electrophoresis showed at least one of the tw...

Journal: :Journal of clinical microbiology 2003
Birgit Strommenger Christiane Kettlitz Guido Werner Wolfgang Witte

In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(A), erm(C) (macrolide-lincosamide-streptogramin B resistance), vat(A), vat(B),...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2006
Dae S Song Bo K Kang Jin S Oh Gun W Ha Jeong S Yang Hyoung J Moon Yong-Suk Jang Bong K Park

A novel multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) that can detect porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine group A rotavirus (GAR) was developed. The 3 viruses (PEDV, TGEV, and porcine GAR) are major agents in viral enteric diseases of piglets. As the clinical signs of these diseases are similar, including...

2015
Christian D. Ahberg Andreas Manz Pavel Neuzil

Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating dyes (such as SYBR Green) into the PCR mixture allowed real-time reaction monitoring and extraction of quantitative information (qPCR). Probes with different excitation spe...

2010
Hyo-Sub Shim Songmi Noh Ae-Ran Park Young-Nam Lee Jong-Kee Kim Hyun-Jae Chung Keum-Soon Kang Nam Hoon Cho

BACKGROUND The aim of this study was to determine the prevalence of human papillomavirus (HPV) and 15 species that cause sexually transmitted infections (STIs) in negative cytology. In addition, we compared the diagnostic performance of multiplex polymerase chain reaction (PCR) with widely available techniques used to detect HPV. METHODS We recruited 235 women of reproductive age who had nega...

2005
Seok Jong Yu Yong Seong Cho Byeong-Jin Jeong Hyeon S. Son Sang Joo Lee

Multiplex PCR method is a powerful technology as a potent tool to amplify specific DNA fragments from genomic DNA using Taq DNA polymerase and primer sets. Since multiple reactions occur in single tube, multiplex PCR has the potential to produce considerable savings of time and effort. Its applications are widely ranged from in vitro cloning of specific regions of DNA to diagnosis of disease [1...

Journal: :The new microbiologica 2010
Giorgio Liguori Francesca Gallé Angela Lucariello Valeria Di Onofrio Luciana Albano Gennaro Mazzarella Maurizio D'Amora Fabio Rossano

This study evaluated the performances of three phenotypic systems (RapID Yeast panel, Vitek2 YST card, and API 20 C AUX) and multiplex PCR for Candida spp. identification. Four-hundred and fifty clinical strains of Candida spp. were identified with the four systems and results of multiplex PCR were compared with those of phenotypic methods. The best correspondence was obtained between Multiplex...

2014
F.L.E. Spositto P.A.Z. Campanerut L.D. Ghiraldi C.Q.F. Leite M.H. Hirata R.D.C. Hirata V.L.D. Siqueira R. Fressatti Cardoso

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

2014
Janine T. Bossé Yanwen Li Øystein Angen Lucy A. Weinert Roy R. Chaudhuri Matthew T. Holden Susanna M. Williamson Duncan J. Maskell Alexander W. Tucker Brendan W. Wren Andrew N. Rycroft Paul R. Langford

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, ...

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