Plant diseases caused by numerous pathogens such as bacteria, viruses, and fungi are responsible for substantial economic losses in the agricultural industry worldwide. Specific, sensitive, efficient diagnostic tools have been developed worldwide to mitigate prevent pathogenic threat. The revolutionized from classical methods more advanced molecular approaches enzyme-linked immunosorbent assay ...

Background and Aims: Infectious mononucleosis (IM)is the clinical manifestation of primary infection with Epstein-Barr virus (EBV). Humans are the only known reservoir of EBV. Regarding the problems in diagnosis of the disease, the purpose of this study was to assess Enzyme-linked immunosorbent assay (ELISA) and Nested polymerase chain reaction (PCR) as a diagnostic tool for this disease. Mate...

BACKGROUND We aimed to develop a PCR-RFLP assay based on available sequences of putative vertebrate hosts to identify blood meals ingested by field female sand fly in the northwest of Iran. In addition, the utility of PCR-RFLP was compared with ELISA as a standard method. METHODS This experimental study was performed in the Insect Molecular Biology Laboratory of School of Public Health, Tehra...

Citation: Boughattas S (2015) Commentary on: “Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran”. Front. Microbiol. 6:215. doi: 10.3389/fmicb.2015.00215 Commentary on: “Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat b...

A prospective study was carried out to evaluate the sensitivity of dengue NS1 antigen-capture ELISA in comparison with dengue virus isolation, conventional RT-PCR and real-time RT-PCR for laboratory confirmation of acute dengue based on single-acute serum samples. Four primary healthcare centres were involved to recruit patients with clinical diagnosis of dengue illness. Patient's demographic, ...

The purpose of the present study was to evaluate efficacy Enzyme-Linked Immunosorbent Assay (ELISA), immunochromatographic (ICG), and reverse transcription-polymerase chain reaction (RT-PCR) methods for detection rotavirus (RV) bovine coronavirus (BCV). Feces samples were collected from 90 diarrhoeic calves (male female) up one month age immune response against RV BCV infection assessed by usin...

Dear Editor, Chronic carriers of hepatitis infection are living throughout the world mostly in the South East of Asia. Recent studies have shown that some HBsAg negative individuals may develop some sort of chronic hepatitis B which is detectable employing some new laboratory diagnosis techniques such as molecular tests. In human during decades, HBV had many mutations by which this virus has be...

We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immun...

Methods Reconstituted human gingival epithelia (RHGE) were challenged with two isoforms of PgLPS together with E. coli LPS as the positive control. mRNA and proteins were harvested from tissues and culture supernatants were collected. Expression of pro-inflammatory and antiinflammatory cytokines was evaluated by Q-PCR and ELISA. Involvement of pattern recognition receptors and signaling pathway...