BACKGROUND Current malaria diagnostic tests, including microscopy and antigen-detecting rapid tests, cannot reliably detect low-density infections. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive but remain too complex for field deployment. A new commercial molecular assay based on loop-mediated isothermal amplification (LAMP) was assessed for field use. METHODS...

Since traditional diagnosis of dermatophyte infections is slow, we present a rapid new PCR test for detection of Trichophyton spp., Microsporum canis and M. audouinii infections. The performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human DNA control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from gu...

Papillary thyroid carcinoma (PTC) is the most common malignant thyroid tumor, and 36-69% of PTC cases are caused by mutations in the BRAF gene. The substitution of a valine for a glutamic acid (V600E) comprises up to 95-100% of BRAF mutations; therefore, most diagnostic methods, including allele-specific PCR and real-time PCR, are designed to detect this mutation. Nevertheless, other mutations ...

This report describes a rapid detection procedure for salmonellae from chicken feces by the combination of tetrathionate primary enrichment (preenrichment [PE])-bacterial lysis-capillary PCR and capillary gel electrophoresis. Pure Salmonella enterica serovar Enteritidis 64K was reisolated and detected by capillary PCR after buffered peptone water and nutrient broth, tetrathionate broth base Haj...

Canine babesiosis is a tickborne, protozoal, haemoparasitic disease. Babesia organisms are frequently classified as either large (B. canis) or small gibsoni). The aim of this study was an attempt to detect B. gibsoni DNA in blood samples taken from dogs suspected suffering tick-borne diseases. 216 were tested using PCR, which, 99 them canisDNA detected, whereas 3 detected. Positive PCR results ...

Five diagnostic techniques performed on skin biopsies (shoulder region) and/or serum were compared for detection of bovine viral diarrhea virus infection in 224 calves 0-3 months of age, 23 calves older than 3 months but younger than 7 months, and 11 cattle older than 7 months. The diagnostic methods used were immunohistochemistry (IHC), 2 commercial antigen ELISAs, 1 commercial antibody ELISA,...

Nanoparticles (NPs) are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications. Photon upconversion is the phenomenon where high-energy photons are emitted upon excitation of low-energy photons. Nucleic acids detection based on upconversion nanoparticles (UCNPs), which display a high signal-to-noise ratio and no p...

The aim of this study was to determine the presence methicillin resistant staphylococci (MRS) in retail raw chicken meat samples sold Hatay. antimicrobial susceptibility isolates tested for 13 different antimicrobials by disc diffusion method and investigated resistance genes encoding (mecA), tetracycline (tetM, tetK), penicillin (blaZ), macrolide (ermA, ermC), lincosamide (lnuA) aminoglycoside...

BACKGROUND & OBJECTIVE Rubella virus (RV) is one of the leading causes of childhood blindness in India. In this study we applied an optimized nested reverse transcription polymerase chain reaction (nRT-PCR) to detect RV in clinical specimens. METHODS nRT-PCR was optimized using total RNA extracted from standard strain of RV using nested sets of primers specific for E1 open reading frame. nRT-...

Cronobacter genus bacteria are food-borne pathogens. Foods contaminated with spp. may pose a risk to infants or immunocompromised adults. The aim of this study was determine the microbiological quality nuts, seeds and dried fruits special emphasis on occurrence Analyses were carried out 64 samples commercial nuts (20 samples), (24), candied (8), (4), mixes seeds, (8). tested for total plate cou...