results escherichia coli phytase was expressed in p. pastoris under different cultivation conditions (post-induction temperature, methanol concentration, and post-induction ph). the optimized conditions by rsm using face centered central composite design were 1% (v/v) methanol, ph = 5.8, and 24.5°c. under the optimized conditions, appa was successfully expressed in p. pastoris and the maximum p...

Glucaric acid is a kind of organic acid and widely used in food, medicine and chemical industries. During the past decade, model microorganisms such as Escherichia coli and Saccharomyces cerevisiae have been engineered for production of glucaric acid. Especially, Pichia pastoris has also been engineered for production of glucaric acid with the highest reported value. In this commentary, recent ...

Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pi...

(2015) High-Level Expression of Endo-β-N-Acetylglu-cosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosyla-tion of Glycoproteins. Copyright: © 2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided t...

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 1...

Abstract Prochiral pyrmetazole can be asymmetrically oxidized into ( S )-omeprazole, a proton pump inhibitor that is used to treat gastroesophageal reflux, by an engineered cyclohexanone monooxygenase (CHMO Acineto - Mut) has high stereoselectivity. CHMO Mut produced heterologous expression in Escherichia coli , where it expressed intracellularly. Thus, isolating this useful biocatalyst require...