We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluoresc...

Projecting from most cell surfaces, cilia serve as important hubs for sensory and signaling processes have been linked to a variety of human disorders, including Bardet-Biedl Syndrome (BBS), Meckel-Gruber (MKS), Nephronophthisis (NPHP), Joubert Syndrome, these diseases are collectively known ciliopathy. DCDC2 is ciliopathy protein that localizes cilia; nevertheless, our understanding the role i...

Caenorhabditis elegans (C. elegans), a member of the phylum Nematoda, carries the evolutionarily conserved genes comparing to mammals. Due to its short lifespan and completely sequenced genome, C. elegans becomes a potentially powerful model for mechanistic studies in human diseases. In this mini review, we will outline the current understandings on C. elegans as a model organism for microRNA (...

Stearic acid represents one of the most abundant fatty acids in Western diet and profoundly regulates health diseases animals human beings. We previously showed that stearic supplementation promoted development terrestrial model nematode Caenorhabditis elegans chemically defined CeMM food environment. However, whether other nematodes remains unknown. Here, we found dietary with could promote ma...

during a survey, five known species of plant-parasitic nematodes of the families tylenchidae and dolichodoridae (tylenchomorpha: tylenchoidea) were collected and identified from several locations in golestan, guilan, kurdistan, and fars provinces. paratrophurus kenanae, tylenchus elegans and t. ritae, as new records for iranian nematode fauna, with the two previously reported species filenchus ...

Protein disulphide isomerase (PDI; EC 5.3.4.1) is a multifunctional polypeptide that is identical to the β subunit of prolyl 4-hydroxylases. We report here on the cloning and expression of the Caenorhabditis elegans PDI}β polypeptide and its isoform. The overall amino acid sequence identity and similarity between the processed human and C. elegans PDI}β polypeptides are 61% and 85% respectively...

The discovery of RNA interference (RNAi) in C. elegans has had a major impact on scientific research, led to the rapid development of RNAi tools and has inspired RNA-based therapeutics. Astonishingly, nematodes, planaria and many insects take up double-stranded RNA (dsRNA) from their environment to elicit RNAi; the biological function of this mechanism is unclear. Recently, the E. coli OxyS non...