نتایج جستجو برای: page protein analysis

تعداد نتایج: 3877308  

Background: Pertussis is still one of the major public health problems. The increase of the disease emerged in recent decades due to the replacement of the reactogenic whole cell vaccine with the safer acellular vaccine and the genetic diversity of the bacterium. As outer membrane vesicles (OMVs) obtained from Bordetella pertussis contains surface immunogenic antigen in its structure, it has an...

Bread’s quality depends on wheat flours quality and quantity and for the goal to be achieved, the usage of suitable wheat varieties should be considered. Present study focuses on analyzing doubled-haploid lines of wheat’s bread backing quality and the relationship between qualitative traits and glutenins reservoir proteins. In current work, traits related to bread backing quality of 30 doubled-...

ژورنال: Vaccine Research 2017

Introduction: Hepatitis E virus (HEV) is a fecal-oral transmitting virus which causes a chronic liver disease. ORF2 is an immunogen capsid protein of HEV that has been proposed to be used for Hepatitis E vaccine design. It is a 660-amino acid protein which includes an immunogenic region (residues 112-607). This protein has been expressed in complete and truncated forms, using different expressi...

Hamid Abtahi, Nafiseh Al-Sadat Mirjamali Neda Molaee Safieh Soufian, Shabnam Sadoogh Abbasian

Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...

Journal: :Electrophoresis 2006
Frank Krause

It is an essential and challenging task to unravel protein-protein interactions in their actual in vivo context. Native gel systems provide a separation platform allowing the analysis of protein complexes on a rather proteome-wide scale in a single experiment. This review focus on blue-native (BN)-PAGE as the most versatile and successful gel-based approach to separate soluble and membrane prot...

Journal: :avicenna journal of medical biotechnology 0

background: bone morphogenetic proteins (bmps) belong to the transforming growth factor-β (tgf-β) superfamily, and play an important role in bone metabolism. recombinant forms of bmp-2 and bmp-7 are the only bmps used clinically. in this study the mature part of human bone morphogenetic protein-7 (bmp-7) was engineered through substitution of the bmp-7 n-terminal sequence by heparin-binding sit...

Journal: :The Biochemical journal 2011
Sari Järvi Marjaana Suorsa Virpi Paakkarinen Eva-Mari Aro

Gel-based analysis of thylakoid membrane protein complexes represents a valuable tool to monitor the dynamics of the photosynthetic machinery. Native-PAGE preserves the components and often also the conformation of the protein complexes, thus enabling the analysis of their subunit composition. Nevertheless, the literature and practical experimentation in the field sometimes raise confusion owin...

Journal: :iranian journal of allergy, asthma and immunology 0
parvin mosadeghi department of biology, basic science faculty, payam noor university of mashhad, mashhad, iran hafez heydari zarnagh molecular and cellular research center, sabzevar university of medical science, sabzevar, iran mohammad mohammad-zadeh molecular and cellular research center, sabzevar university of medical science, sabzevar, iran masoud salehi moghaddam department of biology, basic science faculty, payam noor university of mashhad, mashhad, iran

expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

Journal: :iranian journal of immunology 0
fatemeh malaei biology research center, faculty of science, imam hussein university mahdi hesaraki department of stem cell and developmental biology, cell science research center, royan institute for stem cell biology and technology, acecr, tehran mojtaba saadati biology research center, faculty of science, imam hussein university ali mohammad ahdi department of genetics, faculty of science, shahrekord university, shahrekord mohammad sadraeian pharmaceutical sciences research center, faculty of pharmacy, shiraz university of medical sciences, shiraz, iran hussein honari biology research center, faculty of science, imam hussein university shahram nazarian

background: recombinant vaccine technology is one of the most developed means in controlling infectious diseases. however, an effective vaccine against shigella is still missing. objective: to evaluate recombinant ipac protein of shigella as a vaccine candidate. methods: in this study we cloned ipac gene into an expression vector in prokaryotic system. the protein expression was evaluated by sd...

Journal: Vaccine Research 2015
A Bolhassani , N Davoudi , Sh Irani, SM Mirnurollahi ,

Introduction: Hepatitis C virus (HCV) infection is a major public health problem which influences about 170 million people worldwide. Different types of vaccines have been designed using HCV structural proteins to control the viral infection. The core nucleocapsid protein is one of the most conserved proteins and a desirable target for HCV vaccines, especially the protein-based vaccines. In cur...

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