This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude extract was purified using sequence an aqueous two-phase system (ATPS) in large volume systems (10, 50, 500 g) increase obtaining enzyme, followed diafiltration (DF) step. Purified characterized terms protein profile analysis SDS-PAGE, optimum ...