Isolated mouse liver mitochondria were incubated in two types of P(32)-labelled sucrose-phosphate buffers. The first contained no added ATP or oxidizable substrate. The second contained added ATP. Samples were taken at specified times, up to 60 minutes, and analyses were made of the mitochondrial TCA-soluble inorganic P(32) and the total mitochondrial residue P(31) and P(32). The results of the...

Cysteine oxidation in protamines leads to their oligomerization and contributes to sperm chromatin compaction. Here we identify the Drosophila thioredoxin Deadhead (DHD) as the factor responsible for the reduction of intermolecular disulfide bonds in protamines and their eviction from sperm during fertilization. Protamine chaperone TAP/p32 dissociates DNA-protamine complexes in vitro only when ...

Auchter (1) wras the first to show that very little lateral nmovemiient of nutrient elemiients occurred in certain plants. The work of Caldwell (3) and McAMurtrey (7) also indicate(d a lack of lateral movemleint of mineral elements in the test plants used. From these studies, it seenms that there is a lack of lateral nmovement of nmineral nutrients in some plants. It was (lecidedl to test this ...

Dinitrophenol (DNP) and decanoate acted as uncoupling agents in intact Ehrlich ascites cells. Their effects were exerted very rapidly, and within 5 minutes of their addition to ascites cells all adenosine triphosphate (ATP) and adenosine diphosphate (ADP) was lost from the cells. In the presence of either DNP or decanoate there was a decrease in the extent of labeling of the ATP plus ADP fracti...

T H E nature of the agent which brings about induction during the early development of vertebrates has been extraordinarily difficult to determine. As yet we are not even certain whether there is a passage of a comparatively massive quantity of material from the organizer into the reacting competent tissue, as is probably most usually assumed, or whether the action is primarily located at the s...