We investigate overlap fluctuations of the Sherrington-Kirkpatrick mean field spin glass model in the framework of the Random Overlap Structure (ROSt). The concept of ROSt has been introduced recently by Aizenman and coworkers, who developed a variational approach to the Sherrington-Kirkpatrick model. We propose here an iterative procedure to show that, in the so-called Boltzmann ROSt, Aizenman...

Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio. LATE-PCR generates single-stranded pr...

The invention of polymerase chain reaction (PCR) in 1983 revolutionized many areas of science, due to its ability to multiply a number of copies of DNA sequences (known as amplicons). Here we report on a method to double the throughput of quantitative PCR which could be especially useful for PCR-based mass screening. We concurrently amplified two target genes using only single fluorescent dye. ...

Hybrid gene PML-RARα is the molecular target found in most cases of acute promyelocytic leukemia (APL) and has been used for diagnosis and minimal residual disease studies. The standard molecular technique employed is qualitative reverse transcriptase-polymerase chain reaction (RT-PCR), but with the emergence of real time PCR (Q-PCR), PML-RARα gene detection approaches have been described allow...

In this work we study overlap degrees expressed in terms of overlap functions. We present the basic properties that from our point of view must satisfy these overlap functions. We study a construction method, we analyze which t-norms are also overlap functions and we prove that if we apply particular aggregations to such functions we recover the overlap index between fuzzy sets as defined by Du...

A PCR-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening PCRs and species identification by PCR-enzyme-linked immunosorbent assay. This was applied to DNA extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven region...