Background: Ovarian tissue cryopreservation is a feasible method to preserve female reproductive potential, especially in young patients with cancer or in women at risk of premature ovarian failure. Vitrification has recently emerged as a new trend for biological specimen preservation. On the other hand, gene expression that changes during vitrification can influence oocyte maturation and need ...

BACKGROUND For women facing gonadotoxic treatment, cryopreservation of ovarian tissue with subsequent retransplantation during remission is a promising technique for fertility preservation. However, follicle loss within grafted ovarian tissue can be caused by ischemia and progressive revascularization. Several xenograft models using different immunodeficient rodent lines are suitable for studyi...

Fertility preservation has gained increased acceptance, and many reproductive medicine centers have established programs for this service. A recent report, which collected data from all Nordic university hospitals that had initiated programs for fertility preservation (N = 14), found that breast cancer is the most common diagnosis among women who underwent procedures aimed at fertility preserva...

STUDY QUESTION Can a vitrification protocol using an ethylene glycol/dimethyl sulphoxide-based solution and a cryopin successfully cryopreserve baboon ovarian tissue? SUMMARY ANSWER Our results show that baboon ovarian tissue can be successfully cryopreserved with our vitrification protocol. WHAT IS KNOWN ALREADY Non-human primates have already been used as an animal model to test vitrifica...

Advancements of diagnosis and treatment have substantially improved cancer survival rates in the last few decades. The increasing number of survivors focuses attention on long-term effects caused by cancer treatment and its impact on quality of life. Ovarian failure is one of the major sequelae of cytotoxic chemotherapy and/or radiotherapy in female children and reproductive-age women. Oncologi...

BACKGROUND Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vi...

BACKGROUND Oocyte in vitro maturation (IVM) and cryopreservation at the time of routine ovarian tissue freezing may be offered to cancer patients as an additional option for fertility preservation. This study aimed to investigate the developmental capacity of oocytes isolated from unstimulated ovaries. METHODS Immature oocytes (n = 63) from seven consenting premenopausal patients were analyse...

Despite reasonable achievements in different animal species, the debate about many technical aspects of ovarian tissue banking is continuing. Human ovarian tissue banks are increasingly established around the world without a clear plan about how to make the best use of such tissue. One of the important challenges facing this growing technology is to determine the ideal method for the use of thi...

Background The aim of this study was to investigate the effect of applying the 1-mT magnetic field (MF) in the ovarian tissue vitrification. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice randomly were divided into 5 groups: V1: Ovaries that immediately after leaving of the mice body, were examined histologically (control group), V2: Ovaries that withou...