bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. the aim of this study was to develop a polymerase chain reaction(pcr) test for detection of virulent shigella spp.. for this purpose, the primers were designed to amplify a 526-bp internal region of the shigella spp. icsa gene, which encod...

Objective(s): The porins A and B and also outer membrane vesicles (OMVs) of Neisseria meningitidis are used for vaccine purposes. In the present study, we aimed to design a new vaccine candidate based on a fusion of PorA of serogroups A and B of N. meningitidis admixed with OMV and evaluate it in an animal model.Materials and Methods: Af...

Bacterial surface proteins are involved in direct contact with host cells and in uptake of nutrients from the environment (1). For this reason, cellular localization can provide insights into the functional role of bacterial proteins. Surface localization of bacterial proteins is a key step towards identification of virulence factors involved in mechanisms of pathogenicity. Methods for fraction...

Bacterial outer membrane proteins, along with a filling lipid molecule can be modified to form stable self-assembled monolayers on gold. The transmembrane domain of Escherichia coli outer membrane protein A has been engineered to create a scaffold protein to which functional motifs can be fused. In earlier work we described the assembly and structure of an antibody-binding array where the Z dom...

in this paper, synthesized (raw) multi-walled carbon nanotube (rmwcnts) surfaces were modified with chitosan and β-cyclodextrin using chen’s soft cutting technique. raw and surface modified multi-walled carbon nanotubes were observed by transmission electron microscope (tem). the results showed that chitosan and β-cyclodextrin could attach to the outer surface of nanotubes, wrapping the nanotub...