Background: Equine chorionic gonadotropin (eCG) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. Classical purification of the eCG from serum includes pH fractionation with metaphosphoric acid two ethanol precipitation steps as...

Bacterial wetwood, bacterial dropsy, or slime is a common disease caused by Lelliottia nimipressuralis, which affects the central core of many conifers and deciduous trees. Representatives this species have been isolated from variety trees showing symptoms disease, as well water and, less commonly, clinical samples. Important aspects pathogenesis process pathogen recognition protection mechanis...

constituent of foetal serum, but it virtually disappears from the circulation very soon after birth and is not detectable in normal adults. The only conditions where cx1FP has been found in the sera of adult humans in significant quantities are primary hepatocellular cancer of the liver and teratocarcinomas. It has been shown that the demonstration of x1FP in the serum of adult patients can be ...

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An enzyme-linked immunoabsorbent assay was developed for detection and quantification of the yolk protein lipovitellin (Lv) and its plasma precursor, vitellogenin (Vg), in winter flounder (Pleuronectes americanus). Native Lv was found to be a mixture of heat-stable and heat-labile molecules in mature, ovulated eggs. A heat-stable Lv fraction was purified from extracts of unfertilized eggs by br...

Tyrosine aminotransferase was highly purified from rat livers in which the enzyme had been induced by a glucocorticoid hormone. The preparation eluted from a DEAEcellulose column as a single peak gave a single precipitin line by the Ouchterlony double diffusion precipitin reaction and sedimented as a single boundary in the ultracentrifuge with an s20,W of 5.9. A molecular weight of 91,000 was d...

A method has recently been described (Elek, 1948) for the detection in vitro of toxin-producing organisms including C. diphtheriae. The present paper has two purposes. The first is the examination of a larger series of strains of C. diphtheriae with a view to establishing the reliability of the procedure, for if the method is to be of any practical value for clinical purposes it must demonstrat...

In a previous investigation (Strange and Belton, 1954), a protein fraction which was active in immunizing rabbits against artificially induced anthrax infection was isolated from culture filtrates of Bacillus anthracis. For the production of protective antigen, the synthetic medium of Wright et al. (1954) or a modified medium containing casein hydrolyzate (Belton and Strange, 1954) was used. No...

Activation of the first component of human complement (C1) in human sera can be readily detected in double immunodiffusion studies with anti-C1q, anti- C1r, and anti-C1s as it produces a characteristic pattern quite different from that of precursor C1. Native macromolecular C1 gives a continuous line of precipitation with antisera to C1q, C1r, and C1s in double diffusion studies. After activati...