Isolated rat liver nuclei were washed with Triton-X-100 in the presence of liver cell sap. This treatment liberated a fraction of polysomes which were isolated by differential centrifugation and were designated "outer membrane polysomes." The outer membrane polysomes synthesized protein in vivo. Shortly after injection of orotic acid-(14)C, the RNA of outer membrane polysomes had a higher speci...

Cotton ovules cultured in an insufficiency of boron (10 micromolar), showed inhibition of fiber growth by the ninth day in culture. Averaging data from eight to eleven days of culture under these conditions, total incorporation of [6-(14)C]orotic acid into fiber was inhibited by 59%. Inhibition was evident in all radioactively labeled pools, indicating that the effect may be at the membrane tra...

By CiIAnLI s M. HUGULEY, Jn., JAMES A. BAIN, SHIRLEY L. RIVERS AND ROBERT B. ScoGcINs T HE MAJORITY of anemias associated with a megalohiastic hone marrow respond to vitamin B,2 parenterally. Those which do not respond usually fall into well defined categories of nutritional deficiency, intestinal malahsorption syndromes, pernicious anemia of pregnancy, megaloblastic anemia of infancy or reacti...

The effects of dietary adenine in fatty liver induced by orotic acid (OA) were studied. Rats were paired-fed 1% OA-supplemented diets with/or without 0.25% adenine or a diet without OA for 10 days. Serum lipid profiles were measured using enzyme assay kits. Lipids of liver tissues were extracted and liver lipid contents were determined. A peach of liver was prepared to determine the activities ...

In recent years erotic acid (uracil-4-carboxylic acid) has been found to be essential for the growth of some microorganisms;l some pyrimidine-requiring Neurospora mutants were found to grow equally well with uracil and erotic acid2 and some mutants have been found to produce erotic acid.3 It has been suggested that erotic acid is a by-product in the biosynthesis of pyrimidines in Neurospora.s I...

Rats fed orotic acid develop fatty livers characterized by triglyceride-laden, membrane-bounded vesicles designated "liposomes." We have measured the levels of apolipoproteins in isolated liposomes and other subcellular fractions by SDS-polyacrylamide gel electrophoresis, electrotransfer, and immunodecoration. Apolipoproteins Bh, Bl, E, and C appear to cofractionate; for these proteins, the lip...