objective: organophosphorus hydrolase (oph) is a homodimeric enzyme that can hydrolyze phosphoester bonds and reduce the toxicity of organophosphorus compounds. this makes oph a suitable element for the biodegradation of these compounds. methods: we successfully cloned the oph gene from pseudomonas diminuta, after optimization for pichia pastoris, into a yeast expression vector (ppiczαb). afte...

in this study, the antioxidant activity and protective effects of purslane seed water-methanolic extract (pswme) in stabilizing soybean oil (sbo) were tested. dpph radical scavenging activity of pswme at different concentrations varied significantly (p< 0.05), ranging from 15.41% to 79.06%. total phenolic content in pswme was 121.09 mg gallic acid equivalent (gae) kg-1 dw. the protective effect...

wheat streak mosaic virus (wsmv) causes extensive economic damage to wheat (triticum aestivum l.) in many regions of the world. the present study was conducted to find out if the variations in biochemical changes in reaction to wsmv infection in f2 generation of either potentially resistant (adl-cross) or susceptible (marvdasht) cultivars are genetically inherited. a factorial experiment was em...

a new method for analysis of benzene, toluene, and xylene (btx) using high performance liquid chromatography-uv detection (hplc-uv) is described and compared to the gas chromatography (gc) method. a charcoal adsorption tube connected to a small pump was used to obtain samples from an atmosphere chamber standard. samples were extracted with methanol and analyzed by hplc-uv. chromatography was is...

The results of comparison of experimental C1 NQR parameters for a great number of organic and organometallic compounds and estimated ones using ab initio calculations at the RHF/6-31G(d) level were systematized. The NQR frequency changes on going from one compound to another depend, in general, on the changes of populations of the CI atom p„-orbitals in these compounds and, first of all, of pop...

The synthesis of sterically hindered anilines has been a significant challenge in organic chemistry. Here we report a Cu-catalyzed radical addition with in situ-generated nitroso compounds to prepare sterically hindered amines directly from readily available materials. The transformation is conducted at room temperature, uses abundant copper salts, and is tolerant of a range of functional groups.

The results of earlier investigations of the carcinogenic action of azo dyes in rats were summarized by Kinosita in 1937 and 1940 (22, 23), and by Hartwell in 1941 (17). Since then much work has been done involving various types of controlled, restricted diets and a theory has been evolved (19) to account for the activity of certain dyes and the inactivity of others. A short review of this work...