نتایج جستجو برای: orf2 protein

تعداد نتایج: 1234968  

Journal: :Microbiology 2007
So-ichiro Nishiyama Yukitaka Murakami Hideki Nagata Satoshi Shizukuishi Ikuro Kawagishi Fuminobu Yoshimura

The FimA fimbriae of Porphyromonas gingivalis, the causative agent of periodontitis, have been implicated in various aspects of pathogenicity, such as colonization, adhesion and aggregation. In this study, the four open reading frames (ORF1, ORF2, ORF3 and ORF4) downstream of the fimbrilin gene (fimA) in strain ATCC 33277 were examined. ORF2, ORF3 and ORF4 were demonstrated to encode minor comp...

Journal: :Microbiology 2005
Ching Leang Derek R Lovley

The Fe(III)-reducing micro-organism Geobacter sulfurreducens requires an outer-membrane c-type cytochrome, OmcB, for Fe(III) reduction, but a related cytochrome, OmcC, which is 73 % identical to OmcB, is not required. The omcB and omcC genes are part of a tandem chromosomal duplication consisting of two repeated clusters of four genes. The 2.7 kb sequences preceding omcB and omcC are identical ...

Journal: :Applied and environmental microbiology 1998
B Floriano J L Ruiz-Barba R Jiménez-Díaz

Enterocin I (ENTI) is a novel bacteriocin produced by Enterococcus faecium 6T1a, a strain originally isolated from a Spanish-style green olive fermentation. The bacteriocin is active against many olive spoilage and food-borne gram-positive pathogenic bacteria, including clostridia, propionibacteria, and Listeria monocytogenes. ENTI was purified to homogeneity by ammonium sulfate precipitation, ...

2014
Durga B. Kuchibhatla Westley A. Sherman Betty Y. W. Chung Shelley Cook Georg Schneider Birgit Eisenhaber David G. Karlin

The genome sequences of new viruses often contain many "orphan" or "taxon-specific" proteins apparently lacking homologs. However, because viral proteins evolve very fast, commonly used sequence similarity detection methods such as BLAST may overlook homologs. We analyzed a data set of proteins from RNA viruses characterized as "genus specific" by BLAST. More powerful methods developed recently...

Journal: :Applied and environmental microbiology 2000
K Savijoki A Palva

A tripeptidase (PepT) from a thermophilic dairy starter strain of Lactobacillus helveticus was purified by four chromatographic steps. PepT appeared to be a trimeric metallopeptidase with a molecular mass of 150 kDa. PepT exhibited maximum activity against hydrophobic tripeptides, with the highest activity for Met-Gly-Gly (K(m), 2.6 mM; V(max), 80.2 micromol. min(-1). microg(-1)). Some of the h...

Journal: :Journal of bacteriology 2006
Fumiko Taguchi Yujiro Ogawa Kasumi Takeuchi Tomoko Suzuki Kazuhiro Toyoda Tomonori Shiraishi Yuki Ichinose

Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 dele...

2013
Olga Piskareva Christina Ernst Niamh Higgins Vadim Schmatchenko

The human LINE-1/L1 ORF2 protein is a multifunctional enzyme which plays a vital role in the life cycle of the human L1 retrotransposon. The protein consists of an endonuclease domain, followed by a central reverse transcriptase domain and a carboxy-terminal C-domain with unknown function. Here, we explore the nucleic acid binding properties of the 180-amino acid carboxy-terminal segment (CTS) ...

Journal: :Applied and environmental microbiology 2005
Huseyin Basim Gerald V Minsavage Robert E Stall Jaw-Fen Wang Savita Shanker Jeffrey B Jones

We characterized the copper resistance genes in strain XvP26 of Xanthomonas campestris pv. vesicatoria, which was originally isolated from a pepper plant in Taiwan. The copper resistance genes were localized to a 7,652-bp region which, based on pulsed-field gel electrophoresis and Southern hybridization, was determined to be located on the chromosome. These genes hybridized only weakly, as dete...

1998
SHIRLEY A. WALKER

An inducible middle promoter from the lactococcal bacteriophage f31 was isolated previously by shotgun cloning an 888-bp fragment (P15A10) upstream of the b-galactosidase (b-Gal) gene (lacZ.st) from Streptococcus thermophilus (D. J. O’Sullivan, S. A. Walker, S. G. West, and T. R. Klaenhammer, Bio/Technology 14:82–87, 1996). The promoter showed low levels of constitutive b-Gal activity which cou...

Journal: :Plasmid 2014
Tannaz Jalilsood Ali Baradaran Foo Hooi Ling Shuhaimi Mustafa Khatijah Yusof Raha Abdul Rahim

Lactobacillus plantarum PA18, a strain originally isolated from the leaves of Pandanus amaryllifolius, contains a pR18 plasmid. The pR18 plasmid is a 3211bp circular molecule with a G+C content of 35.8%. Nucleotide sequence analysis revealed two putative open reading frames, ORF1 and ORF2, in which ORF2 was predicted (317 amino acids) to be a replication protein and shared 99% similarity with t...

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