Acridine orange is a metachromatic intercalator used extensively in histochemistry to differentiate double- from single-stranded (ds, ss) nucleic acid by the emission of green and red fluorescence, respectively, under ultraviolet light. In the present study we standardised a protocol in order to use acridine orange to detect rotavirus ds RNA in polyacrylamide gels and compared it to silver and ...

BACKGROUND Vitamin D has been added to calcium-fortified orange juice. It is unknown whether vitamin D is as bioavailable from orange juice as it is from supplements. OBJECTIVES The objective was to compare the bioavailability of vitamin D(2) and vitamin D(3) from orange juice with that from vitamin D(2) and vitamin D(3) supplements. A secondary aim was to determine which form of vitamin D is...

A 31-year-old atopic subject with allergic rhinoconjunctivitis with sensitization to several pollens, presented with urticaria and angioedema after ingestion of orange blossom pollen (Citrus sinensis). A positive skin prick test for orange blossom pollen extract (BIAL-Aristegui, Bilbao, Spain) at a concentration of 1.2 mg/ml was obtained. Serum specific IgE antibodies to orange blossom pollen w...

This study evaluated the effect of foliar biostimulants on fruit quality of adult 'Valencia' orange and 'Biancolilla' olive trees. Half of the selected orange and olive trees were sprayed twice before harvest with the SUNRED® commercial mix containing phenylalanine, methionine, mono-saccharides and oxylipins from plant extracts as well as potassium salts and urea. Orange and olive yields and fr...

Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. Sweet orange accounts for more than half of the Citrus production both in fresh fruit and processed juice. We have sequenced the draft genome of a double-haploid sweet orange (C. sinensis cv. Valencia), and constructed the Citrus sinensis annotation proj...

Spina bifida is a birth defect that affects the spines of developing fetuses and infants, and research in the 20th century indicated that chemicals in the herbicide Agent Orange likely led to the birth defect. People with spina bifida [6] can have nerve damage, paralysis, and mental disabilities. During the Vietnam War in the 1960s, the US military employed Agent Orange and other herbicides to ...

because of the everyday use of power generators in iraq, which exist in residential neighborhoods, it is necessary to study the impact on soils and plants surrounding areas of those generators. lead is one the most heavy metals released especially, as a result of the combustion of diesel fuel during operation of the powered generators. however, a study was conducted to investigate the lead conc...

background and purpose: generated dye wastewater by the textile industry is usually toxic, non-biodegradable and resistant in the environment. eggshell is one of the inexpensive material and for the reason the vesicular structures can be used as a proper adsorbent for pollutants removal. the aim of this study is to investigate the efficiency of eggshell for removal of acid orange 2 dye from aqu...

introduction: the aim of this in vitro study was to evaluate the dissolving efficacy of eucalyptol and orange oil solvents associated with passive ultrasonic activation (pua) in zinc oxide-eugenol (zoe) based and epoxy resin-based root canal sealers. methods and materials: seventy samples of each sealer were prepared and then randomized according to the solvent and the time of the ultrasonic ac...