نتایج جستجو برای: optimal gene expression
تعداد نتایج: 1949057 فیلتر نتایج به سال:
The bioavailability of methylmercury (MeHg) in the brains of orange spotted groupers, captured from four creeks of Mahshahr embayment was measured. Then the effects of this pollutant on the regulation of gene expression, acetylcholinesterase transcript levels was chosen in order to monitor the amounts of methylmercury concentrations in the creeks, and the fluctuations of mRNA expressions in t...
BACKGROUND Dedifferentiation of chondrocytes remains a major problem for cartilage tissue engineering. Chondrocytes loss differentiated phenotype in in vitro culture that is undesired for repair strategies. The chondrocyte is surrounded by a pericellular matrix (PCM), together forming the chondron. PCM has a positive effect on the maintenance of chondrocyte phenotype during culture in compar...
Introduction: Omentin, an adipokine, with anti-inflammatory effects reduces insulin resistance, and can hence, play an important role in prevention of cardiovascular disease and diabetes. The present study aimed to investigate the association of plasma and dietary fatty acids with gene expression of omentin in visceral and subcutaneous adipose tissues. Materials and Methods: Visceral and subcut...
The gene encoding the human basic fibroblast growth factor (hbFGF) has been already chemically-synthesized and cloned in pET-3a expression vector (Pasteur Institute of Iran). In the present study, we compared the level of expression of this synthetic hbFGF and its related cDNA in Escherichia coli. The pBR322-cDNA of hbFGF supplied by Dr. Seno (from Molecular Biology Dept, Okaido prefectural uni...
Background : Skeletal muscles are composed of various contracted fibrils, which are mainly divided into fast-twitch and slow-twitch. This study aimed to investigate 8 weeks endurance activity on the MEF2 and HDACA4 gene expression in fast-twitch and slow-twitch skeletal muscles in male Wistar rats. Materials and Methods: in order to carry out this study, 20 heads of male Wistar rat...
Background: Vaccines have played a special role in controlling and reducing mortality from infectious diseases. In this regard, DNA vaccines were developed to ease the production and reduce the risks of traditional vaccines. Human papillomavirus (HPV) has been introduced as the causing agent of cervical cancer. The capsid protein (L1) of HPV has been used to produce subunit and DNA vaccines. Th...
background dedifferentiation of chondrocytes remains a major problem for cartilage tissue engineering. chondrocytes loss differentiated phenotype in in vitro culture that is undesired for repair strategies. the chondrocyte is surrounded by a pericellular matrix (pcm), together forming the chondron. pcm has a positive effect on the maintenance of chondrocyte phenotype during culture in compariso...
background bone marrow and adipose tissue are two main sources of mesenchymal stem cells (mscs). some of studies suggest that there are some differences in gene expression profile of mscs-derived from various tissues. to investigate gene expression profile by qrt-pcr, an appropriate reference gene with stable expression level should be chosen for normalizing data. this study was designed to ev...
most plants encounter stress such as drought and salinity that adversely affect growth, development and crop productivity. the expression of the gene glutathione-s-transferases (gst) extends throughout various protective mechanisms in plants and allows them to adapt to unfavorable environmental conditions. gstf1 (the first phi gstfs class) gene expression patterns in the wheat cultivars mahuti ...
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