abstract background: fertilization failure is one of the most problems in assisted reproduction technology (art). objective: the aim of this study was the evaluation of oocytes activation by addition of calcium ionophore in unfertilized oocytes in icsi cycles. materials and methods: this study was done on 15 icsi cycles (stimulated with standard long protocol). mature retrieved oocytes with nor...

background: vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. the objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. methods: oocytes were either cryopreserved as immature cumulus compact (imcc) (n=98 exp 1; ...

Intercellular bridges first appear during lizard oogenesis when follicles are rather small (150 microgram in diameter); at this stage they form connecting links between the oocyte and follicle cells, which have not yet differentiated into pyriform cells. Later on, when the follicles have become larger (1 mm) and the follicular epithelium appears constituted by 3 types of cells (small, intermedi...

Introduction: Genital system is important herd in animal's creation. It is necessary for animal reproduction. Mammal has two sex in genital system such as male and female in separated animals. Animal reproduction destroyed by disorder of genital system. In this case, animals cannot produce an animal similar to them. Finally it cause overthrow a species of animals. Methods: Histomorpholog...

A new cell surgery technique has been developed to produce well-defined alterations in cells and tissue without detectable heating and/or other structural damage in the surroundings. The technique involves the use of an argon fluoride excimer laser, in the deep ultraviolet (UV) region of the spectrum at 193 nm, which is guided through a glass pipette filled with a positive air pressure. To demo...

BACKGROUND The aim of this study was to assess the effect of an individualized GnRH antagonist regimen on folliculogenesis. METHODS In a multicentre, randomized, clinical trial, IVF/ICSI patients were allocated to a standard regimen, in which they received daily 0.25 mg GnRH antagonist ganirelix (Orgalutran) from the 6th day of stimulation onward (fixed regimen n = 102) or to an individualize...

The present study describes the analysis of the associations between the growth hormone gene polymorphism (Leu/Val) and oocyte maturation and in vitro fertilisation in cattle. Two independent experiments were carried out. In the first one, oocytes were collected from 49 single ovaries, matured in vitro, measured and cytogenetically analysed. One ovary was considered as a donor. The procedure of...

OBJECTIVE To evaluate whether the surgical resection or aspiration with ethanol sclerotherapy (AEST) of endometrioma before in vitro fertilization (IVF) affect controlled ovarian stimulation (COS) and IVF outcome in the infertilie women with endometroma undergoing IVF. METHODS In this retrospective cohort study, 101 consecutive IVF/intracytoplasmic sperm injection cycles that were performed i...

The purpose of this study was to evaluate the effectiveness cysteamine supplementation on maturation medium and/or in vitro fertilization with regards improving normal rate sheep oocytes, which are characterized by formation two pronuclei. Grade A and B oocytes were matured medium-199 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), human chorionic gonadotrophin (hC...