Out of 120 fecal samples collected from diarrheic calves at different localities in AL-Qalyoubia Governorate. 43 were positive for Escherichia coli with an incidence of 35.83%.Serogrouping of E.coli isolates recovered 8 belong to different "O" serogroups which were O55, O111, O26, O153, O8, O18, O86, O157, and "5" isolates were untypable. Antibiogram pattern of isolated E.coli serogroups showed...

During the wild boars hunting season (2012-2013), 6508 adults were shot in Lazio Region, Viterbo territory. Out of the 6508, 393 subjects were sampled to be tested for the detection of Salmonella spp., Yersinia enterocolitica, E. coli VTEC and Trichinella spp. For this purpose, diaphragm and skeletal muscles samples were collected after the shooting, and tested at the Istituto Zooprofilattico S...

Molecular detection of bacterial pathogens based on LAMP methods is a faster and simpler approach than conventional culture methods. Although different LAMP-based methods for pathogenic bacterial detection are available, a systematic comparison of these different LAMP assays has not been performed. In this paper, we compared 12 in-house real-time LAMP assays with a commercialized kit (Isotherma...

Background: E. coli is one of the most important zoonotic pathogen and also an indicator faecal contamination food water. There paucity data regarding level raw meat characterization pathogenic from slaughtered cattle chicken in un-organized sector Mizoram, India. Methods: Raw samples traditionally were collected Aizawl, Kolasib Champhai districts Mizoram analyzed for count (ECC), serotypes vir...

Cattle manure is one of the primary larval developmental habitats of house flies, Musca domestica (L.). Cattle serve as asymptomatic reservoirs of Shiga toxin-producing Escherichia coli (STEC), and bacteria are released into the environment in cattle feces. The USDA-FSIS declared seven STEC serogroups (O157, O26, O45, O103, O145, O121, and O111) as adulterants in beef products. In addition, the...

Escherichia coli O157:H7 and certain non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups have emerged as important public health threats. The development of methods for rapid and reliable detection of this heterogeneous group of pathogens has been challenging. GeneDisc real-time PCR assays were evaluated for detection of the stx(1), stx(2), eae, and ehxA genes and a gene that iden...

Bacteriological analyses of seawater from three main beaches in Fortaleza, Brazil were performed during 1997. Thirty-six samples per beach were collected for a total of 108 samples. For Meireles Beach, 44% of the samples had MPN total coliforms values of at least 1100 or over 2400/100 ml, followed by Formosa and Diários beaches showing lower counts. For fecal coliforms the highest numbers were ...

During a 2009 nationwide outbreak of sorbitolfermenting Escherichia coli O157 in Norway, the Norwegian Institute of Public Health was notified of diarrhoea outbreaks in two nurseries. A link to the nationwide outbreak was suspected and investigated, including retrospective cohort studies. Both nurseries had recently visited farms. Faecal specimens were obtained from symptomatic children as well...

This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 1...