Northern blot analysis of nucleic acid from potato plant tissues and tobacco protoplasts infected with a Scottish isolate of potato leafroll luteovirus (PLRV) detected the 6 kb genomic RNA and one subgenomic RNA species of about 2.7 kb; RNA extracted from virus particles contained only the genomic species. Blotting with small defined probes suggested that the location of the 5' end of the subge...

Changes in nucleotides and ribonucleic acids (RNA) in 'Valencia' orange have been determined as influenced by storage, ethylene, heat and commercial concentrating practices. All treatments tended to lower polymeric RNA species. Patterns of interactions in the case of nucleotides were evident and distinct for certain treatments. Changes in nucleotides may be related to the quality of the process...

We describe methods for the complete analysis of cellular nucleotides from as few as 10(6) 32Pi-labeled cells in a simple 2-day experiment. Nucleotides are extracted with acid, neutralized, and resolved by two-dimensional thin layer chromatography on polyethyleneimine cellulose. In the first dimension the nucleotides are separated based on the negative charge of their phosphate groups (i.e. cyc...

With the discovery of ATP structure in 1929, significant progress was made understanding role nucleosides and nucleotides body. One most important breakthroughs is associated with determination function an autacoid ATP, a participant purinergic signal transmission. For first time, this pointed out by Professor Geoffrey Burnstock 1972. Purinergic signaling activators are extracellular including ...

Previously, we characterized two host protein binding elements located within the 3'-terminal 166 nucleotides of the mouse hepatitis virus (MHV) genome and assessed their functions in defective-interfering (DI) RNA replication. To determine the role of RNA secondary structures within these two host protein binding elements in viral replication, we explored the secondary structure of the 3'-term...

The interaction between nucleotides and carbon nanotubes (CNTs) is a subjectof many investigations for treating diseases but there are many questions in this field thatremain unanswered. Because of experimental methods involve assumptions andinterpretation besides limitations, there are many problems that the best study for them isusing theoretical study. Consequently, t...

objective: one of the most significant damages to the genetic material is oxidative deamination of dna and free nucleotides in the cell pool. incorporation of deaminated purine nucleotides such as inosine triphosphate (itp, ditp) into the dna can increase the frequency of base substitution mutation. it has been suggested that presence and accumulation of these rough nucleotides can lead to gene...

Besides being a metabolite of nucleotides like ATP, adenosine is a mediator of neuronal function in the central nervous system. Its actions are mediated by at least three extracellular receptors. In this review different aspects of adenosine such as biosynthesis, release, inactivation and its receptors are discussed. It also covers pre- and postsynaptic effects as well as postreceptor mecha...