introduction: hepatitis c virus (hcv) infection is a major public health problem which influences about 170 million people worldwide. different types of vaccines have been designed using hcv structural proteins to control the viral infection. the core nucleocapsid protein is one of the most conserved proteins and a desirable target for hcv vaccines, especially the protein-based vaccines. in cur...

Background: With the rapidly increasing incidence of novel coronavirus disease 2019 (COVID-19) and detection severe acute respiratory syndrome 2 (SARS-CoV-2) RNA in plasma, blood supply safety has become a main concern. Objectives: Due to some reports on RNAemia SARS-CoV-2-infected donors, this study examined presence SARS-CoV-2 asymptomatic donors. Methods: In cross-sectional study, about 400 ...

Alanine substitution mutations in the Sendai virus nucleocapsid (NP) protein have defined highly conserved hydrophobic and charged residues from amino acids (aa) 362 to 371 that are essential for function of the protein in RNA replication. Mutant NP362, which had the change F362A, was incapable of supporting in vitro RNA replication. NP362 expressed alone formed extended oligomers which exhibit...

Severe acute respiratory syndrome coronavirus 2 poses a huge threat to humans. The β genus has four structural proteins: Spike, envelope, membrane and nucleocapsid protein. Among them, spike plays an important role in the host cell attachment fusion. S1 subunit of is responsible for bonding hence most crucial target finding appropriate inhibitors. Molecular docking calculations at receptor bind...

Purified intact Sindbis virus nucleocapsids were treated at different pH values or with various concentrations of divalent cations, cation chelators, salt, or formamide. The resulting structures were examined by velocity sedimentation, electron microscopy, and protein-protein cross-linking. Changes in each of the test conditions led to alterations in the sedimentation profile of treated nucleoc...