The incorporation in vivo of compounds labeled with N15 or Cl4 into the purines and pyrimidines of nucleic acid has been repeatedly demonstrated (e.g. (l-3)). W e wish to report observations on the incorporation of radioactive erotic acid in vitro into the pyrimidines of the nucleic acid of rat liver slices. It is believed that this procedure will readily permit the study of precursors of nucle...

Abstract Somaclonal variation during in vitro culture is often an undesirable phenomenon but may also be a source of genetic useful for breeders. The molecular mechanisms underlying this remain uncertain. In study, we analyzed the fruit transcriptome three independent cucumber lines using RNA-sequencing technology and performed extensive silico study to determine how somaclonal altered gene exp...

Abstract Nucleic acids are one of the most important cellular components. These molecules have been studied both experimentally and theoretically. As all-atom simulations still limited to short time scales, coarse-grain modeling allows study those on a longer scale. Nucleic-Acid united RESidue (NARES-2P) is low-resolution coarse-grained model with two centers interaction per repeating unit. It ...

DNA-DNA hybridization (DDH) has been used for nearly 50 years as the gold standard for prokaryotic species circumscriptions at the genomic level. It has been the only taxonomic method that offered a numerical and relatively stable species boundary, and its use has had a paramount influence on how the current classification has been constructed. However, now, in the era of genomics, DDH appears ...

The nucleic acid content of growing normal and pathological tissues has been a subject of interest in recent years. Several investigators (l-7) have studied nucleic acid changes during embryonic development. It is a well established fact that rapidly growing embryonic tissue has a high concentration of nucleic acid. This was recently extended by Reddy and Cerecedo (S), who observed that gestati...

We analyze the scaling and cost-performance characteristics of current and projected connectomics approaches, with reference to the potential implications of recent advances in diverse contributing fields. Three generalized strategies for dense connectivity mapping at the scale of whole mammalian brains are considered: electron microscopic axon tracing, optical imaging of combinatorial molecula...

Analyzing EGFR mutations and detecting ALK gene fusion are indispensable when planning to treat pulmonary adenocarcinoma. Malignant pleural effusion (MPE) is a devastating complication of lung cancer and sometimes the only source for mutation analysis. The percentage of tumor cells in the pleural effusion may be low; therefore, mutant enrichment is required for a successful analysis. The EGFR m...

We analyze the scaling and cost-performance characteristics of current and projected connectomics approaches, with reference to the potential implications of recent advances in diverse contributing fields. Three generalized strategies for dense connectivity mapping at the scale of whole mammalian brains are considered: electron microscopic axon tracing, optical imaging of combinatorial molecula...

The more than three decades of research in nucleic acid nanotechnology has led to the thrilling progress rationally designed structures and artificial molecular devices with programmable functions various applications. Nucleic acid–based aggregates feature precise recognition sequence programmability, versatility, as well marked biocompatibility, providing promising candidates for biomedical In...