نتایج جستجو برای: ns5a protein

تعداد نتایج: 1235289  

Journal: :Journal of virology 2014
Shali Shi Xiaoyan Yu Yimin Gao Binbin Xue Xinjiao Wu Xiaohong Wang Darong Yang Haizhen Zhu

Hepatitis C virus (HCV) core protein is essential for virus assembly. HCV core protein was expressed and purified. Aptamers against core protein were raised through the selective evolution of ligands by the exponential enrichment approach. Detection of HCV infection by core aptamers and the antiviral activities of aptamers were characterized. The mechanism of their anti-HCV activity was determi...

2010
Vlastimil Jirasko Roland Montserret Ji Young Lee Jérôme Gouttenoire Darius Moradpour Francois Penin Ralf Bartenschlager

Non-structural protein 2 (NS2) plays an important role in hepatitis C virus (HCV) assembly, but neither the exact contribution of this protein to the assembly process nor its complete structure are known. In this study we used a combination of genetic, biochemical and structural methods to decipher the role of NS2 in infectious virus particle formation. A large panel of NS2 mutations targeting ...

2012
Shuanghu Liu Li Xiao Cassie Nelson Curt Hagedorn

The unique properties of the hepatitis C virus (HCV) JFH1 isolate have made it possible to produce and study HCV in an infectious cell culture system. However, relatively low virus titers restrict some of the uses of this system and preparing infectious chimeric reporter viruses have been difficult. In this study, we report cell culture-adapted mutations in wild-type JFH1 yielding higher titers...

2018
Ryuichi Sugiyama Asako Murayama Sayuri Nitta Norie Yamada Megumi Tasaka-Fujita Takahiro Masaki Hussein Hassan Aly Masaaki Shiina Akihide Ryo Koji Ishii Takaji Wakita Takanobu Kato

The number of amino acid substitutions in the interferon (IFN) sensitivity-determining region (ISDR) of hepatitis C virus (HCV) NS5A is a strong predictor for the outcome of IFN-based treatment. To assess the involvement of ISDR in the HCV life cycle and to clarify the molecular mechanisms influencing IFN susceptibility, we used recombinant JFH-1 viruses with NS5A of the genotype 1b Con1 strain...

2014
Yimin Gao Xiaoyan Yu Binbin Xue Fei Zhou Xiaohong Wang Darong Yang Nianli Liu Li Xu Xiaohong Fang Haizhen Zhu

NS2 protein is essential for hepatitis C virus (HCV) replication. NS2 protein was expressed and purified. Aptamers against NS2 protein were raised and antiviral effects of the aptamers were examined. The molecular mechanism through which the aptamers exert their anti-HCV activity was investigated. The data showed that aptamer NS2-3 inhibited HCV RNA replication in replicon cell line and infecti...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس 1387

مقدمه: ویروس هپاتیت c یکی از عوامل اصلی ایجاد بیماری مزمن کبدی و سرطان کبد است. درمان ترکیبی با پگ اینترفرون و آنالوگ نوکلئوزیدی ریباویرین در بسیاری از بیماران منجر به پاکسازی ویروس می شود. یکی از ژن های القاء شده توسط اینترفرون ، پروتئین کیناز r است. تاثیر ضد ویروسی این پروتئین به علت تعامل با یکی از عوامل شروع ترجمه به نام eif2? و در نتیجه ممانعت از سنتز پروتئین می باشد. به نظر می رسد پروتئین...

Journal: :Topics in antiviral medicine 2017
David L Wyles Anne F Luetkemeyer

Viral resistance to direct-acting antiviral drugs may impact their effectiveness during treatment of hepatitis C virus (HCV) infection. Most data on HCV drug resistance concern genotypes 1 and 3. The clinical impact of resistance to HCV nonstructural protein 5A (NS5A) inhibitors and a practical approach to indications and methods for resistance testing are discussed.

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