the objective of this study was to explore the microbial community diversities and the relationships between microbial community compositions and hydrochemical factors in nitrate contaminated groundwater of hun river alluvial plain. the method of polymerase chain reaction (pcr)-denaturing, gradient gel electrophoresis (dgge) gene fingerprints combined with canonical correspondence analysis（cca）...

functionalization of multiwalled carbon nanotubes (mwcnts) with nh2 groups under a one pot reaction is studied. during the first step of the reaction, cl and chcl2 groups were attached to the surfaces of mwcnts through an electrophilic addition reaction. in the second step of process, cl atoms were replaced with nh2 and amino groups (ethylene diamine and butyl amine) under a nucleophilic substi...

Immune precipitates were solubilized by the alternative pathway of complement assembled from isolated proteins, i.e., C3, factor B, factor D, properdin, C3b inactivator (C3bINA), and beta 1H. The kinetic curves of solubilization in the isolated system and in EGTA-serum were virtually indistinguishable. No requirement of other factors was apparent. Deletion of C3bINA and beta 1H from the complet...

The phenomenon of detergent insolubility of bovine hippocampal membranes in Triton X-100 was monitored by estimating the presence of phospholipids in the insoluble pellet. This represents a convenient and unambiguous assay and reports the dependence of the extent of phospholipid solubilization on detergent concentration. The advantage of this approach is its ability to accurately determine the ...

BACKGROUND Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and organic solvents like n-propanol have been successfully used as mild solubili...