نتایج جستجو برای: nmr spectrometer

تعداد نتایج: 79900  

Journal: :International journal of molecular medicine 2014
Valeria Righi Yiorgos Apidianakis Nikolaos Psychogios Laurence G Rahme Ronald G Tompkins A Aria Tzika

In vivo nuclear magnetic resonance spectroscopy (NMR), a non-destructive biochemical tool used for investigating live organisms, has recently been performed in studies of the fruit fly Drosophila melanogaster, a useful model organism for investigating genetics and physiology. We used a novel high-resolution magic angle-spinning (HRMAS) NMR method to investigate live Drosophila GST2 mutants usin...

Journal: :Solid state nuclear magnetic resonance 2012
Kazuyuki Takeda

Recent reports on microcoils are reviewed. The first part of the review includes a discussion of how the geometries of the sample and coil affect the NMR signal intensity. In addition to derivation of the well-known result that the signal intensity increases as the coil size decreases, the prediction that dilution of a small sample with magnetically inert matter leads to better sensitivity if a...

Journal: :The European respiratory journal 2012
A Sinha V Krishnan T Sethi S Roy B Ghosh R Lodha S Kabra A Agrawal

By using an Anacon condenser (Biostec, Valencia, Spain), different results have been reported [7]. Collection devices are an important source of variability of EBC biomarkers [2, 3, 8]. The principal variability factors include cooling temperature [9] and condenser materials [2]. For example, a warm-up during condensation is observed when RTube or Anacon are used (condensers using a disposable ...

2004
E. Barbero Soto D. Cornuet B. Dehning M. Hildreth J. Matheson G. Mugnai A. Müller E. Peschardt M. Placidi J. Prochnow F. Roncarolo P. Renton E. Torrence P. S. Wells J. Wenninger G. Wilkinson

The determination of the centre-of-mass energies for all LEP 2 running is presented. Accurate knowledge of these energies is of primary importance to set the absolute energy scale for the measurement of the W boson mass. The beam energy between 80 and 104 GeV is derived from continuous measurements of the magnetic bending field by 16 NMR probes situated in a number of the LEP dipoles. The relat...

2017
Sarantos Kostidis Daria Kokova Natalia Dementeva Irina V. Saltykova Hye Kyong Kim Young Hae Choi Oleg A. Mayboroda

BACKGROUND Analysis of the stool samples is an essential part of routine diagnostics of the helminthes infections. However, the standard methods such Kato and Kato-Katz utilize only a fraction of the information available. Here we present a method based on the nuclear magnetic resonance spectroscopy (NMR) which could be auxiliary to the standard procedures by evaluating the complex metabolic pr...

Journal: :The Journal of Cell Biology 1982
R J Gillies T Ogino R G Shulman D C Ward

The phenomenon of intracellular pH (pHin) regulation in cultured Ehrlich ascites cells was investigated using 31P nuclear magnetic resonance (NMR) spectroscopy. Measurements were made with a Bruker WH 360 wide bore NMR spectrometer at a 31P frequency of 145.78 MHz. Samples at a density of 10(8) cells ml-1 were suspended in a final volume of 2 ml of growth medium in 10 mm diameter NMR tubes. Int...

Journal: :Journal of magnetic resonance 2000
M Andrec K G Inman D J Weber R M Levy G T Montelione

It has recently become more widely appreciated that the presence of rotational diffusional anisotropy in proteins and other macromolecules can have a significant affect on the interpretation of NMR relaxation data in terms of molecular motion. In this paper, we show how commonly used NMR relaxation data (R(1), R(2), and NOE) obtained at two spectrometer frequencies can be analyzed using a Bayes...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2005
Gaohua Liu Yang Shen Hanudatta S Atreya David Parish Ying Shao Dinesh K Sukumaran Rong Xiao Adelinda Yee Alexander Lemak Aneerban Bhattacharya Thomas A Acton Cheryl H Arrowsmith Gaetano T Montelione Thomas Szyperski

A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five G-matrix Fourier transform NMR experiments for resonance assignment based on highly resolved 4D and 5D spectral information is acquired in conjunction with a single simultaneous 3D 15N,13C(aliphatic...

2007
Steven C. McGie Daniel P. Hallihan Enid Pehowich Christopher Hanstock Peter S. Allen Vivian K. Mushahwar

The goals of this study were: to determine whether Nuclear Magnetic Resonance NMR spectroscopy could produce decipherable spectra of the metabolites in the spinal cord even when small volumes of tissue are used, and to assess the spatio-temporal changes in metabolite concentrations following spinal cord injury. Adult rats received a severe spinal cord injury at the 8th thoracic level. Spinal co...

Journal: :Journal of agricultural and food chemistry 2001
Z Xia L G Akim D S Argyropoulos

Novel protocols for acquiring quantitative (13)C NMR spectra of lignins have been developed using the internal reference compounds 1,3,5-trioxane and pentafluorobenzene. Trioxane offers a convenient internal standard for collecting inverse gated proton decoupled (13)C NMR spectra for lignins, whereas pentafluorobenzene can be used to provide information on the amount of methine carbon using the...

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