The green synthesis of silver nanoparticles is a rapid and environmentally friendly technique (AgNPs). Silver ions are bioreduced into AgNPs by compound found in Skimmiaanquetilia's aqueous leaf extract, which consists primarily alkaloids coumarins. were synthesized characterized multiple techniques, including ultraviolet-visible spectroscopy, Fourier transform infrared analysis, scanning elect...

After discovering that toluene 4-monooxygenase (T4MO) of Pseudomonas mendocina KR1 oxidizes nitrobenzene to 4-nitrocatechol, albeit at a very low rate, this reaction was improved using directed evolution and saturation mutagenesis. Screening 550 colonies from a random mutagenesis library generated by error-prone PCR of tmoAB using Escherichia coli TG1/pBS(Kan)T4MO on agar plates containing nitr...

The human aryl sulfotransferases HAST4 and HAST4v vary by only two amino acids but exhibit markedly different affinity towards the sulfonate acceptor p-nitrophenol and the sulfonate donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). To determine the importance of each of these amino acid differences, chimeric constructs were made of HAST4 and HAST4v. By attaching the last 120 amino acids of HA...