A variety of different compounds of fused uracils were prepared simply by the heating of 6-hydrazinyl-1-methyl-, 6-hydrazinyl-1-propyl-, or 6-hydrazinyl-1,3-dipropyluracil under reflux with ninhydrin, isatin, benzylidene malononitrile, benzylylidene ethyl cyanoacetate, benzil, and phenacyl bromide derivatives. The newly synthesized compounds were completely screened for antimicrobial and antitu...

1. Rat heart was perfused with a mixture of amino acids and other ninhydrin-positive substances in the same concentrations as they are found in plasma, and with or without sufficient puromycin to suppress protein synthesis all but completely. 2. In the presence of puromycin, but not in its absence, insulin increased the concentrations of most of the individual amino acids in the intracellular w...

The endosperm of castor bean seeds (Rkinus comunuis L.) contains two -SH-dependent amptidases, one hydrolyzing L-leucine-,Bnaphthylamide optimally at pH 7.0, and the other hydrolyzing L-proline,B-naphthylamide optimally at pH 7.5. After germination the endosprm contains in addition an -SH-dependent hemoglobin protease, a serinedependent carboxypeptidase, and at least two -SH-dependent enzymes h...

A fllwrometric amino acid analyzer using fllmresramine for the assay of the full array of natural amino ac<ids including prolinc on a single column is reported. The proline determination was carried out by specific introduction of a solution of ,\‘chlorosl~ccinimide into the flow system. Single rolumn fluorometric amino acid analysis was curried out in a significantly shorter time and with a se...

We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 mumol of alpha-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automate...

high voltage electrophoresis. The average yield of the glycopeptide was in the range of 0.2 to 0.3 mg/liter of urine. Sugar analysis and amino acid analysis gave equimolar amounts of glucose, fucose, and threonine. Linkages and sequential order were established by methylation analysis of the glycopeptide after degradation of the amino acid residue with ninhydrin. The permethylated product was a...