A total of 350 staphylococci isolated from various clinical sources were examined for bound and free coagulase, fermentation of mannitol, and deoxyribonuclease. The economical coagulase-mannitol-agar method of Esber and Faulcomer was found to be suitable for the detection of free coagulase and mannitol fermentation. A significant number of coagulase- and mannitol-negative staphylococci proved t...

Molecular determination of carriage of the mecA locus in coagulase negative staphylococci in screening swabs from patients in an intensive care unit Staphylococci are important nosocomial pathogens, which are easily transmitted from patient to patient, and there is concern over the recent emergence of antibiotic resistance to the β lactam class of antimicrobial drugs, particularly within methic...

conclusions: high percentage of hemolysins, biofilm and beta lactamase production by isolated staphylococci, suggests an important role of these virulence factors in the pathogenesis of isolated staphylococci from dermatitis lesions. the s. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics. only ciprofloxacin was found to be active against all is...

We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9 % of the isolates tested to species level. The Vitek MS cor...

discussion our results indicated that agar screening was superior to oxacillin disk agar diffusion. a comparison between the antibiotic sensitivity patterns of the mrcns and the methicillin-susceptible coagulase-negative staphylococci (mscns) showed that the mrcns were predominantly multiple-drug resistant isolates as the simultaneous resistance rate to 4 or more antibiotics in the mrcns and ms...

Bovine mastitis is one of the major ill-health of cattle and buffaloes, responsible for great economic loss to dairy industry affecting livestock farmers worldwide. The major economic consequences of clinical bovine mastitis are due to treatment, loss in milk production, culling, changes in product quality and the risk of other diseases (Halasa et al., 2007). For etiological diagnosis of bovine...

Lysostaphin sensitivity was evaluated as a rapid screening test to differentiate Staphylococcus aureus from other species of staphylococci and micrococci. A total of 168 strains of staphylococci, 108 of which were S. aureus, were cultured overnight in brain infusion broth. Gram stains were peformed before and after a 1:10 dilution of the culture was exposed to 2 micrograms of lysostaphin per ml...

A total of 299 isolates of gram-positive, catalase-positive, coagulase-negative cocci were isolated from a variety of specimens collected from patients at a large university hospital, and 281 (94%) were identified as staphylococci by established methods. Using the scheme of Kloos and Schleifer, we determined the species of the coagulase-negative staphylococci. Staphylococcus epidermidis was the...

Coagulase-negative staphylococci produce an exocellular glycolipid antigen which has potential as a serological marker of infection in bone. The value of this newly detected antigen was investigated by enzyme-linked immunosorbent assay (ELISA) in 15 patients with culture-proven infection of prostheses caused by Gram-positive bacteria. The antigen was purified by gel-permeation chromatography fr...