CONTEXT The diagnostic algorithm for human allergic disease involves confirmation of sensitization by detection of allergen-specific immunoglobulin E (IgE) antibody in individuals suspected of having allergic disease because of a history of allergic symptoms after known allergen exposure. Previous studies showed wide disparity among clinically reported allergen-specific IgE levels from differen...

BACKGROUND Asthma-associated morbidity is rising, especially in inner city children. OBJECTIVE We evaluated the allergen sensitivities, allergen exposures, and associated morbidity for participants in the Inner City Asthma Study. We also determined geographic variations of indoor allergen levels. METHODS Nine hundred thirty-seven inner city children 5 to 11 years old with moderate to severe...

Repeated low-dose allergen challenge increases airway hyperresponsiveness in atopic asthmatics. However, it is not known whether low-dose allergen challenge increases airway inflammation. Eight atopic asthmatics were enrolled in a controlled, cross-over study to evaluate the effect and time course of repeated low-dose allergen challenge on airway inflammation and hyperresponsiveness. The dose o...

Molecular-based allergy (MA) diagnostics is an approach used to map the allergen sensitization of a patient at a molecular level, using purified natural or recombinant allergenic molecules (allergen components) instead of allergen extracts. Since its introduction, MA diagnostics has increasingly entered routine care, with currently more than 130 allergenic molecules commercially available for i...

STUDY OBJECTIVE To examine the baseline factors influencing the occurrence and magnitude of immediate- and late-phase responses in asthmatic patients after an allergen-induced bronchial provocation test (A-BPT). DESIGN Cross-sectional analysis in a homogenous group of 31 mild, Dermatophagoides pteronyssinus-allergic patients with asthma. SETTING Allergy Department, Hospital Virgen del Camin...

BACKGROUND Beech and oak pollen are potential allergen sources with a world-wide distribution. OBJECTIVE We aimed to characterize the allergen profile of beech and oak pollen and to study cross-reactivities with birch and grass pollen allergens. METHODS Sera from tree pollen-allergic patients with evidence for beech and oak pollen sensitization from Basel, Switzerland, (n=23) and sera from ...

Recent evidence shows that natural CD25(+)Foxp3(+) regulatory T cells (nTreg) and induced CD25(+)Foxp3(+) regulatory T cells (iTreg) both contribute to tolerance in mouse models of colitis and asthma, but there is little evidence regarding their relative contributions to this tolerance. We compared the abilities of nTreg and iTreg, both from OVA-TCR-transgenic OTII mice, to mediate tolerance in...

the efficacy of allergen immunotherapy for the treatment of allergic rhinoconjunctivitis with or without seasonal bronchial asthma and anaphylaxis caused by the sting of the hymenoptera class of insects has been clearly demonstrated in numerous well-designed, placebo-controlled trials. immunotherapy whether by subcutaneous injection of allergen extract or by oral/sublingual routes modifies peri...

BackgroundThe interaction of allergens and allergen-specific IgE initiates the allergic cascade after crosslinking receptors on effector cells. Antibodies other isotypes may modulate such a reaction. Receptor requires binding antibodies to multiple epitopes allergen. Limited information is available complexity epitope structure most allergens.ObjectivesWe sought allow description IgE, IgG4, IgG...

objective(s):sublingual allergen-specific immunotherapy is a safe and effective method for treatment of ige-mediated respiratory allergies; however, the underlying mechanisms are not fully understood. this study was planned to test whether sublingual immunotherapy (slit) can exert epigenetic mechanisms through which the airway allergic responses can be extinguished. materials and methods:balb/c...