Prestin, a member of the solute carrier family 26, is expressed in the basolateral membrane of outer hair cells. This protein provides the molecular basis for outer hair cell somatic electromotility, which is crucial for the frequency selectivity and sensitivity of mammalian hearing. It has long been known that there are abundantly expressed approximately 11-nM protein particles present in the ...

OpcA is an integral outer membrane from the Gram-negative pathogen Neisseria meningitidis that plays a role in adhesion of meningococci to host cells. The protein was overexpressed in Escherichia coli in an insoluble form and a procedure developed for refolding by rapid dilution from denaturant into detergent solution. The refolded material was identical to native OpcA isolated from meningococc...

This Earth of Mankind by Pramoedya Ananta Toer is a historical novel about Dutch colonialism in Indonesia. paper was aimed to find and describe the social stratification past that written novel. carried out with only one research question: How Toer’s Novel?. qualitative research, specifically document analysis. The main source this Mankind, 9th edition publishes Hasta Mitra 2002. total page 405...

Assimilation of nitrogen is an essential biological process for plant growth and productivity. Here we show that three chloroplast enzymes involved in nitrogen assimilation, glutamate synthase (GOGAT), nitrite reductase (NiR) and glutamine synthetase (GS), separately assemble into distinct protein complexes in spinach chloroplasts, as analyzed by western blots under blue native electrophoresis ...

There are two types of network traffic in experimental environments. One is traffic which is derived from target elements and the other one is background traffic which is derived from surrounding elements. There is very little knowledge on the relationships between new elements and existing elements; therefore, surrounding elements that seem to have no apparent relationship with the target elem...

This article reports key findings from the first phase of a research project investigating Net generation age students as they encounter e-learning at five universities in England. We take a critical view of the idea of a distinct generation which has been described using various terms including Net generation and Digital Natives and explore age related differences amongst first year university...

Indonesia possesses tremendous marine resources. Therefore, their products are appropriate for exploration. In the prior study, bacteria generating keratinase enzyme have isolated from local fish market trash. The may hydrolyze keratin on skin. Surrounding parameters, such as temperature, pH, and incubation duration, factors affecting activity of enzyme. This study aims to isolate characterize ...

Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...