There is considerable evidence for the essential role of surface water in protein function and structure. However, it is unclear to what extent the hydration water and protein are coupled and interact with each other. Here, we show by ESR experiments (cw, DEER, ESEEM, and ESE techniques) with spin-labeling and nanoconfinement techniques that the vitrified hydration layers can be evidently recog...

Physical characterization of nanoparticles is required for a wide range of applications. Nanomechanical resonators can quantify the mass of individual particles with detection limits down to a single atom in vacuum. However, applications are limited because performance is severely degraded in solution. Suspended micro- and nanochannel resonators have opened up the possibility of achieving vacuu...

8 ABSTRACT: We present the first detailed experimental 9 observation and analysis of nanoparticle electrophoresis 10 through a nanochannel obtained with synchronous high11 bandwidth electrical and camera recordings. Optically 12 determined particle diffusion coefficients agree with values 13 extracted from fitting electrical transport measurements to 14 distributions from 1D Fokker−Planck diffu...

A plasma model of relaxation of a medium in heavy-ion tracks in condensed matter is proposed. The model is based on three assumptions: the Maxwell distribution of plasma electrons, localization of plasma inside the track nanochannel, and constant values of the plasma electron density and temperature during the x-ray irradiation. The model of multiple ionization of target atoms by a fast project...

Several types of nanofluidic devices based on nanopores and nanochannels have been reported to yield ionic current rectification, with the aim to control the delivery of chemical species in integrated systems. However, the rectifying properties obtained by existing approaches cannot be altered once the devices are made. It would be desirable to have the ability to modulate the predefined proper...

A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglycine.

Highly confined fluid or particle flows typically show strongly non-Newtonian behavior. In this study, the relation between the non-Newtonian behavior and the level of confinement is studied. Molecular dynamics simulations, in the canonical ensemble (i.e. the number of particles, volume and temperature are constant), are used to calculate various physical properties. Here we only present the de...

A theory is presented of the longitudinal dispersion of DNA under equilibrium confined in a nanochannel. Orientational fluctuations of the DNA chain build up to give rise to substantial fluctuations of the coil in the longitudinal direction of the channel. The translational and orientational degrees of freedom of the polymer are described by the Green function satisfying the usual FokkerPlanck ...

Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the...

We present an experimental study of nanoscale electrokinetic transport in custom-fabricated quartz nanochannels using quantitative epifluorescence imaging and current monitoring techniques. One aim is to yield insight into electrical double layer physics and study the applicability of continuum theory to nanoscale electrokinetic systems. A second aim is to explore a new separation modality offe...