To examine the relationship between body mass index, blood pressure, and the Na+,K+-adenosine triphosphatase (ATPase) system, we measured the erythrocyte ghost Na+,K+-ATPase and the erythrocyte Na+ concentration in 120 blacks and 127 whites (136 males and 111 females). Blacks showed a 13.9% higher erythrocyte Na+ (7.63 +/- 0.19 vs 6.70 +/- 0.11 [SEM] mEq/L; p = 0.0001) and a 16.1% lower erythro...

Biochemical studies have demonstrated the presence of ouabain-sensitive Na-K-ATPase in lens epithelium. The development of certain experimental cataracts has been associated with the decrease in the level of activity of this enzyme. Cytochemical investigations at the ultrastructural level to localize this important enzyme in the ocular lens are needed to exhibit the site of Na-K-ATPase action. ...

Sznajder, J. I., P. Factor, and D. H. Ingbar. Invited Review: Lung edema clearance: role of Na -K -ATPase. J Appl Physiol 93: 1860–1866, 2002. 10.1152/japplphysiol.00022.2002.—Acute hypoxemic respiratory failure is a consequence of edema accumulation due to elevation of pulmonary capillary pressures and/or increases in permeability of the alveolocapillary barrier. It has been recognized that lu...

To clarify the suppressant action of diphenylhydantoin (DPH) on digitalis-induced arrhythmias we studied effects of DPH and digoxin, alone and in combination, on contractile force, sodium-potassium adenosinetriphosphatase (Na + -K ATPase) activity, and potassium (K) balance in dog hearts perfused with Krebs-Ringer's solution. Neither control perfusion nor DPH alone (3 X 10"M) altered Na + -K AT...

AIMS Na(+), K(+)-ATPase activity contributes to the regulation of vascular contractility and it has been suggested that vascular Na(+), K(+)-ATPase activity may be altered during the progression of diabetes; however the mechanisms involved in the altered Na(+), K(+)-ATPase activity changes remain unclear. Thus, the aim of the present study was to evaluate ouabain-sensitive Na(+), K(+)-ATPase ac...

Here we show that ouabain-induced cell growth regulation is intrinsically coupled to changes in the cellular amount of Na/K-ATPase via the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway. Ouabain increases the endocytosis and degradation of Na/K-ATPase in LLC-PK1, human breast (BT20), and prostate (DU145) cancer cells. However, ouabain stimulates the PI3K/Akt/m...

BACKGROUND We have reported that digitalis-like substances (cardiotonic steroids), including marinobufagenin (MBG), induce endocytosis of the plasmalemmal Na/K-ATPase in LLC-PK1 cells. The current report addresses the potential relevance of plasmalemmal Na/K-ATPase redistribution to in vivo salt handling. METHODS Male Sprague-Dawley rats were given 1 week of a high salt (4.0% NaCl) or normal ...

inhibition convincingly to increased vascular smoothmuscle tone. Furthermore, any hypothesis which attempts to relate such a substance to the pathogenesis of essential hypertension must take into account, in addition to the problems outlined above, the fact that while there is consistent evidence of inhibition of Na+ ,K+-ATPase in the leucocytes of patients with essential hypertension, the body...

Microsomes prepared from electric organ of Electrophorus electricus contain, in addition to a sodiumand potassiumactivated adenosine triphosphatase, two different ATP-ADP transphosphorylases; one requires only magnesium, while the other requires Mg++ + Na+. The Mg++-activated nucleotide exchange is nonspecific with respect to substrates and is probably unrelated to the highly specific Na+-K+ATP...

We tested the hypothesis that previously demonstrated gender differences in ACh-induced vascular relaxation could involve diverse Na(+)-K(+)-ATPase functions. We determined Na(+)-K(+)-ATPase by measuring arterial ouabain-sensitive 86Rb uptake in response to ACh. We found a significant increase of Na+ pump activity only in aortic rings from female rats (control 206 +/- 11 vs. 367 +/- 29 nmol 86R...