RNA labeled with [methyl-3H] methionine and [14C]uridine was isolated from the cytoplasm of adenovirus-infected cells and purified by poly(U)-Sepharose chromatography and hybridization to filters containing immobilized adeovirus DNA. Analysis by dimethyl sulfoxide-sucrose gradient sedimentation suggested that the major mRNA species were methylated. 7-Methylguanosine was identified at the 5'-ter...

The genes encoding Δ6 desaturase, Δ6 fatty acid elongase, and Δ5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Arabidopsis thaliana under the control of the Brassica napus napin promoter to produce arachidonic acid (ARA; 20:4 Δ 8, 11, ) and eicosapentaenoic acid (EPA; 20:5 Δ 8, 11, 14, ). ARA and EPA accumulated up to 0.5% and 0.05% of total fatty acids, respectively...

Methods for the detection of RNA modifications are of fundamental importance for advancing epitranscriptomics. N6 -methyladenosine (m6 A) is the most abundant RNA modification in mammalian mRNA and is involved in the regulation of gene expression. Current detection techniques are laborious and rely on antibody-based enrichment of m6 A-containing RNA prior to sequencing, since m6 A modifications...

The role of Helicobacter pylori urease in opsonization by human complement was investigated. H. pylori wild type strain N6 and isogenic mutants lacking either the large urease subunit (UreB) or an accessory urease protein (UreG) were incubated with different sera. C3b bound to the bacteria was measured by specific staining and flow cytometry. As compared with opsonization of N6 and the UreG-lac...

n3 polyunsaturated fatty acids have been reported to have anti-carcinogenic effects on mammalian carcinomas. n3 fatty acids occur in high concentrations in marine oils, especially shark liver oils. Several reports have indicated an extremely low incidence of cancer in sharks, although other reports indicate carcinogenesis in some shark species. It has been hypothesised that n3 fatty acids and o...

By using immunoelectron microscopy, we have localized the binding site on 50S Escherichia coli ribosomal subunits for puromycin, an antibiotic that interacts with the ribosomal peptidyltransferase center. This was achieved by affinity-labeling 50S subunits with N-bromoacetyl puromycin and treating the labeled subunits with an antibody specific for the N6,N6-dimethyladenosine moiety of puromycin...

We present an algorithm for isolating the roots of an arbitrary complex polynomial p that also works for polynomials with multiple roots provided that the number k of distinct roots is given as part of the input. It outputs k pairwise disjoint disks each containing one of the distinct roots of p, and its multiplicity. The algorithm uses approximate factorization as a subroutine. In addition, we...

ABSTRACT: Peach palm is a domesticated commercially important for the production of fruits and hearts palm. Somatic embryogenesis, an effective technique mass propagation, was successfully established this species. Furthermore, temporary immersion system improved plant regeneration. However, can be further by understanding peach palm’s growth dynamic modifications culture media. The aims study ...