A triple-resonance pulse scheme is described which records(15)N, NH correlations of residues that immediately follow amethyl-containing amino acid. The experiment makes use of a(15)N, (13)C and fractionally deuterated proteinsample and selects for CH(2)D methyl types. The experiment isthus useful in the early stages of the sequential assignment process as wellas for the confirmation of backbone...

In a previous paper (1) we have reported studies on the percentage of N-15 excreted over a twenty-four hour period following the oral administration of N-15 glycine to normal subjects and to patients with Cushing's syndrome in nitrogen equilibrium on diets containing approximately one gram of protein per kilogram of body weight. During this study it was noted that the level of protein intake in...

In the course of our chemical screening program for novel metabolites by LC-MS monitoring, we isolated a new aspochracin derivative, JBIR-15 (1), together with aspochracin, from the culture broth of a sponge-derived fungus, Aspergillus sclerotiorum Huber Sp080903f04. The structure of 1 was determined to be N-demethyl aspochracin at the alanyl residue on the basis of extensive NMR and MS analyses.

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We describe a magic-angle spinning NMR experiment for selective (13)C-(15)N distance measurements in uniformly (13)C,(15)N-labeled solids, where multiple (13)C-(15)N and (13)C-(13)C interactions complicate the accurate measurement of structurally interesting, weak (13)C-(15)N dipolar couplings. The new experiment, termed FSR (frequency selective REDOR), combines the REDOR pulse sequence with a ...

Spectroscopic factors and asymptotic normalization coefficients ~ANCs! have been determined for bound states in O using the N(He,d)O reaction. These results are used to calculate the astrophysical S factor for direct capture in the N(p ,g)O reaction. We also discuss how uncertainties in optical-model parameters influence both the spectroscopic factors and the ANCs, and the effect that this has ...

I was very honored to receive the University of California, San Francisco, and the International Society of Pharmacometrics Lewis Sheiner lecturer award in May 2013. In the present perspective, I outline the main points of my lecture at the American Conference of Pharmacometrics (slides in Supplementary Material 1). I first emphasize the scientific contributions of Lewis Sheiner as a quantitati...