the aim of this study was to clone the serine alkaline protease-encoding gene from bacillus subtilis 168. this protease, which can have many applications especially in detergent, may be industrially an important enzyme. for the amplification of the gene, pcr was performed with a pair of primers specifically designed for this purpose. electrophoresis of the pcr product showed the expected band o...

the purpose of this study was to investigate the polymorphism of k-casein (k-cn), β-lactoglobulin (b-lg) and leptin (lp) genes in iranian holstein cattle by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique. dna was extracted from blood samples of 139 cows using a modified phenol chloroform method. association between k-cn, b-lg and lp genes’ polymorphism w...

Background: In this study we aimed at detecting the enterotoxin A and B gene of S. aureus in clinical samples of patients attending health centers in Zahedan using molecular methods. Materials and Methods: A cross-sectional study was carried out in which 40 samples of S. aureus were obtained from patients in a hospital in Zahedan, Iran. Following the biochemical tests, Identifications were c...

endo-derived nitric oxide (no) is synthesized from l-arginine by endothelium nitric oxide synthase (enos). since reduced no synthesis has been implicated in the development of coronary atherosclerosis; we hypothesized that polymorphisms of nos gene might be associated with increased susceptibility to this disorder and coronary artery disease (cad). we studied the 27 base pair tandem repeat poly...

background: interest in tatera indica rodent arises mostly because it is believed that this species is survived among four subspecies reported from iran, two of which exist in khuzestan province. in addition, it might has a role as res­ervoir hosts of zoonotic cutaneous leishmaniasis in the transmission of leishmania major in some of the widespread asian foci including southwestern iran. method...