نتایج جستجو برای: multiplex rt pcr
تعداد نتایج: 209630 فیلتر نتایج به سال:
BACKGROUND A diversifying pool of mammalian-adapted influenza A viruses (IAV) with largely unknown zoonotic potential is maintained in domestic swine populations worldwide. The most recent human influenza pandemic in 2009 was caused by a virus with genes originating from IAV isolated from swine. Swine influenza viruses (SIV) are widespread in European domestic pig populations and evolve dynamic...
We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PM...
Malaria is a serious public health problem worldwide. Globally concerted efforts are underway to control and eliminate it. Despite recent slowdown, substantial achievements have been recorded in the last 20 years. However, its eradication requires successful elimination of all Plasmodium parasites among symptomatic, asymptomatic, sub-microscopic infections. This review aimed at assessing...
Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the detection and differentiation of pestiviruses. For this purpose, one primer pair was selected from a highly conserved region of the genome of pestiviruses. Using these primers (PEST 1-PEST 2), DNA fragments of between 72 and 74 bp could be amplified from all pestivirus isolates tested. In order to differe...
چکیده اسفناج (spinacia oleracea l.) یکی از مهمترین سبزیهای برگی است که به صورت تازه و یا فرآوری شده مصرف میشود و به دلیل داشتن خواص دارویی و مواد غذایی فراوان به صورت گسترده در دنیا کشت و مصرف میشود. گیاه اسفناج همواره تحت تاثیر بیمارگرهای مختلف قارچی، باکتریایی و ویروسی قرار میگیرد. در نمونهبرداریهای صورت گرفته از مزارع منطقه داوران شهرستان رفسنجان در سال1390، مجموعاً 177 نمونه از گیاها...
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