objective: in order to overcome the limitation of systemic administration of methylene blue, this study investigated the encapsulation of methylene blue in polymeric liposomes and drug release following sonication. methods: we encapsulated methylene blue into nanoliposomes. the dynamic light scattering (dls) method was used to measure the size distribution of the liposomes. after loading methyl...

combination formulations of liposomes with collagen have been previously introduced as a means of obtaining more stable and less permeable liposomes. in this study, the effect of aqueous solutions of the collagen products collapur? (col) and collapuron-dak? (cold) from henkle co., on the release rate of sodium chromate (chr) as a water-soluble model drug from stable plurilamellar vesicles (splv...

PURPOSE Immunotherapy is the most effective treatment against metastatic renal cell carcinoma (RCC). However, the response rate is approximately 15%. More effective therapy is, therefore, needed for patients with metastatic RCC. We then examined the antitumor effect of cationic multilamellar liposome containing human IFN-beta (huIFN-beta) gene (IAB-1) against RCC. EXPERIMENTAL DESIGN Concentr...

Intranasal vaccination is particularly a striking route for mucosal immunization, due to the ease of administration and the induction of both mucosal and humoral immunity. However, soluble antigens (Ag) are not sufficiently taken up after the nasal administration and need to be co-administered with adjuvants, penetration enhancers or encapsulated in particles. So, in this study, tetanus toxoid ...

The current methods for treatment of cancers are inadequate and more specific methods such as gene therapy are in progress. Among different vehicles, cationic liposomes are frequently used for delivery of genetic material. This investigation aims to prepare and optimize DOTAP cationic liposomes containing an antisense oligonuclotide (AsODN) against protein kinase C alpha in non-small cells lung...

The influence of temperature and ionic strength on the vesiculation properties of large multilamellar vesicles containing various proportions of dimyristoylphosphatidylglycerol has been investigated. It is shown that at low ionic strengths preformed large multilamellar vesicles composed of dimyristoylphosphatidylcholine and dimyristoylphosphatidylglycerol (7:3) on incubation at the gel to liqui...

Large unilamellar vesicles (LUVs) composed of 1-[2H31]palmitoyl-2-oleoyl phosphatidylcholine (POPC-d31), with diameters of approximately 117 +/- 31 and 180 +/- 44 nm, were prepared by extrusion through polycarbonate filters with pore sizes of 0.1 and 0.2 microns, respectively. The 2H nuclear magnetic resonance (NMR) spectra obtained at 21 degrees C contain two components: a broad component (app...

Localization of 2,4-dichloroand 2,4-dibromophenol in multilamellar vesicles in a 1/1 dihalogenated phenol/lipid molar ratio was investigated by classical contrast variation X-ray scattering using the isomorphous replacement method. The results were compared with those obtained by anomalous small-angle X-ray scattering from a vesicle system doped with 2,4-dibromophenol. Dissimilarities in the re...