Label-free Raman microspectroscopy combined with a multivariate curve resolution (MCR) analysis can be a powerful tool for studying a wide range of biomedical molecular systems. The MCR with the alternating least squares (MCR-ALS) technique, which retrieves the pure component spectra from complicatedly overlapped spectra, has been successfully applied to in vivo and molecular-level analysis of ...
Journal:
:International journal of molecular medicine2009
Ira KokovicBarbara Jezersek NovakovicSnjezana Frkovic GrazioSrdjan Novakovic
Follicular lymphoma (FL) is characterized by the t(14;18)(q32;q21) chromosomal translocation which can be detected by polymerase chain reaction (PCR) in approximately 70% of cases. The aim of our retrospective study was to evaluate the sensitivity and the reproducibility of both conventional qualitative and quantitative PCR assays for detection of the t(14;18)(q32;q21) chromosomal translocation...
V Mallikarjuna RaoP Mahesh KumarD RambabuRavikumar KapavarapuS Shobha RaniParimal MisraManojit Pal
A series of novel alkynyl substituted 3,4-dihydropyrimidin-2(1H)-one (DHPM) derivatives were designed, synthesized and evaluated in vitro as potential inhibitors of chorismate mutase (CM). All these compounds were prepared via a multi-component reaction (MCR) involving sequential I2-mediated Biginelli reaction followed by Cu-free Sonogashira coupling. Some of them showed promising inhibitory ac...
Nguyen Vinh TrungSébastien MatamorosJuan J. Carrique-MasNguyen Huu NghiaNguyen Thi NhungTran Thi Bich ChieuHo Huynh MaiWillemien van RooijenJames CampbellJaap A. WagenaarAnita HardonNguyen Thi Nhu MaiThai Quoc HieuGuy ThwaitesMenno D. de JongConstance SchultszNgo Thi Hoa
We investigated the consequences of colistin use in backyard chicken farms in Vietnam by examining the prevalence of mcr-1 in fecal samples from chickens and humans. Detection of mcr-1-carrying bacteria in chicken samples was associated with colistin use and detection in human samples with exposure to mcr-1-positive chickens.
Escherichia coli (E. coli) strains placed in predominant nosocomial bacteria intensive care units (ICUs), resulting severe drug-resistant infections. Non-susceptibility to β-lactams and last-line drugs such as Fosfomycin Colistin cause limited availability of infections eradication. The objective this study included the determination genes encoding extended-spectrum β-lactamase (ESBL), Carbapen...
A catalyst, solvent, work-up and column free synthesis of chromenopyranpyrazoles via multicomponent cascade reaction has been achieved with high stereoselectivity. This novel reaction creates two N-C, two C-C and one O-C bonds through a domino process for the construction of three new rings and three contiguous stereogenic centers.
Potassium tribromide wrapped around polyethylene glycol ({K+PEG400}Br3-) was easily prepared by two step reaction of PEG400 with KBr followed by the addition of molecular bromine to give PEG embedded potassium tribromide as a dark viscous oil. The synthesized material was found to be an efficient, cost effective, environmentally benig...
A mild and efficient one-pot three-component and environmentally benign approach for the synthesis of a wide range of hydantoin annulated derivatives has been described. A multi-component reaction between a carbonyl compounds (ketone or aldehyde), potassium cyanide and ammonium carbonate (as cyanating agent and amine source, respectively leads to the formation of hydantoins. The proposed optimi...
Passerini three-component reaction and multicomponent polymerization (MCP) were demonstrated to be facile methods for the preparation of photo-cleavable polymers, photo- and redox-dually cleavable polymers, as well as block copolymers.
