Bacteriophage Mu is a highly efficient transposon which requires the products of the Mu A and B genes in order to transpose at a normal frequency. We have determined the nucleotide sequence of the B gene as well as that of the A-B intergenic region upstream of B. The protein product of the gene contains 312 amino acids and has a predicted molecular weight of 35,061. As expected, there do not ap...

A tetramer of the Mu transposase (MuA) pairs the recombination sites, cleaves the donor DNA, and joins these ends to a target DNA by strand transfer. Juxtaposition of the recombination sites is accomplished by the assembly of a stable synaptic complex of MuA protein and Mu DNA. This initial critical step is facilitated by the transient binding of the N-terminal domain of MuA to an enhancer DNA ...

Transactivator protein C is required for the expression of bacteriophage Mu late genes from lys, I, P and mom promoters during lytic life cycle of the phage. The mechanism of transcription activation of mom gene by C protein is well understood. C activates transcription at Pmom by initial unwinding of the promoter DNA, thereby facilitating RNA polymerase (RNAP) recruitment. Subsequently, C inte...

The title isomorphous compounds, tetra-mu-but-2-enoato-bis[diaqua(but-2-enoato)dysprosium(III)]-2,6-diaminopurine (1/2), [Dy(2)(C(4)H(5)O(2))(6)(H(2)O)(4)].2C(5)H(6)N(6), and tetra-mu-but-2-enoato-bis[diaqua(but-2-enoato)holmium(III)]-2,6-diaminopurine (1/2), [Ho(2)(C(4)H(5)O(2))(6)(H(2)O)(4)].2C(5)H(6)N(6), consist of [Ln(crot)(3)(H(2)O)(2)](2) dimers (crot is crotonate or but-2-enoate; Ln is ...

The endomorphins are recently discovered endogenous agonists for the mu-opioid receptor (Zadina et al., 1997). Endomorphins produce analgesia; however, their role in other brain functions has not been elucidated. We have investigated the behavioral effects of endomorphin-1 in the globus pallidus, a brain region that is rich in mu-opioid receptors and involved in motor control. Bilateral adminis...

Introduction: Transcranial alternating current stimulation (tACS) is emerging as an interventional tool to modulate different functions of the brain, potentially by interacting with intrinsic ongoing neuronal oscillations. Functionally different intrinsic alpha oscillations are found throughout the cortex. Yet it remains unclear whether tACS is capable of specifically modulating the somatosenso...

The stability of delivery of low monitor unit (MU) setting is important especially for step-andshoot intensity-modulated radiotherapy (IMRT), because the nature of the technique is inherent to repeat beam on/off according to the number of the segments. This study evaluates the dose linearity and profile flatness/symmetry under low MU settings for Vero4DRT, a new linear-accelerator based irradia...

The bacteriophage Mu transposase (the Mu A gene product), which is absolutely required for both integration of Mu and replicative transposition during the lytic cycle, has been overproduced by cloning the gene on a plasmid under the control of the phage lambda PL promoter. The protein has been purified to near homogeneity from the lysate of heat-induced cells of a strain carrying the plasmid. T...

The emergence of drug-resistant parasites is a serious threat faced by malaria control programs. Understanding the genetic basis of resistance is critical to the success of treatment and intervention strategies. A novel locus associated with antimalarial resistance, ap2-mu (encoding the mu chain of the adaptor protein 2 [AP2] complex), was recently identified in studies on the rodent malaria pa...

BACKGROUND AND OBJECTIVE Among children's hospitals, little is known about how barriers to electronic health record (EHR) adoption are related to meaningful use (MU) incentives. We investigated hospital success with MU incentive payments and determined associations with hospital-reported challenges and characteristics. METHODS A survey administered to 224 Children's Hospital Association hospi...